民猪猪繁殖与呼吸综合征恢复力高低组血清代谢组学研究  

Study on Serum Metabolomics of High and Low Resilience Group of Min Pigs with Porcine Reproductive and Respiratory Syndrome

作　　者：孟祥旭 李佳[1,2] 任德明 陈奎蓉 和艺云 王立贤 盛熙晖[1] 王立刚[2] MENG Xiangxu;LI Jia;REN Deming;CHEN Kuirong;HE Yiyun;WANG Lixian;SHENG Xihui;WANG Ligang(Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2025年第4期1689-1699,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家生猪产业技术体系(CARS-35)。

摘　　要：旨在对民猪猪繁殖与呼吸综合征(蓝耳病)恢复力高低组血清代谢组学进行研究,为蓝耳病的防控提供参考。本研究从代谢组学的角度出发,选择28日龄民猪15头,体重相近、饲喂环境相同且攻相同蓝耳病毒,从中选取高恢复力组5头、低恢复力组5头,基于液相色谱-质谱(LC-MS)对血清样本进行非靶向代谢组学检测,通过正交偏最小二乘判别分析(OPLS-DA)、聚类分析等筛选不同恢复力民猪血清差异代谢物并进行通路富集分析。结果表明:在不同恢复力组间可筛选得到39个差异代谢物(P<0.05),进一步注释分析发现,鞘磷脂、靛玉红、花生四烯酸、L-苏氨酸可以作为潜在标志代谢物候选。通路分析发现,缬氨酸、亮氨酸和异亮氨酸的生物合成、花生四烯酸代谢通路等可能通过影响炎性细胞因子水平和炎症介质的产生来减轻炎症反应,最终影响恢复力。综上所述,本研究不仅筛选到鞘磷脂、靛玉红、花生四烯酸等蓝耳病恢复力相关标志代谢物,还在代谢物角度初步诠释了恢复力差异高低的可能机制,为蓝耳病的防控和高恢复力民猪选育提供了理论参考。The aim of this study was to study the serum metabolomics of high and low resilience groups of porcine reprodective and respiratory syndrome(PRRS,blue ear disease)in Min pig,and to provide reference for the prevention and control of blue ear disease.In this study,from the perspective of metabolomics,15 Min pigs aged 28 days with similar body weights were selected,the same feeding environment,and the same exposure to porcine reproductive and respiratory syndrome virus(PRRSV).Among them,5 pigs were classified into the high-resilience group and 5 into the low-resilience group.Non-targeted metabolomic analysis of serum samples was performed using liquid chromatography-mass spectrometry(LC-MS).Differential metabolites in the serum of Min pigs with different resilience levels were screened through orthogonal partial least squares discriminant analysis(OPLS-DA)and cluster analysis,followed by pathway enrichment analysis.The results identified 39 differential metabolites between resilience groups(P<0.05).Analysis revealed that sphingolipids,indigo carmine,arachidonic acid,and L-threonine were potential biomarkers.Further analysis indicated that pathways related to valine,leucine,isoleucine biosynthesis,and arachidonic acid metabolism might alleviate inflammatory responses by modulating cytokine levels and inflammatory mediators,thereby affecting resilience.In summary,this study identified sphingomyelin,indigo,arachidonic acid,and other metabolites associated with resilience to blue ear disease.Additionally,it provided a preliminary interpretation of the mechanisms underlying differences in resilience from a metabolic perspective.This research offers theoretical insights for both the prevention and control of blue ear disease and the breeding of Min pigs with high resilience.

关 键 词：民猪 猪繁殖与呼吸综合征 恢复力 代谢组学 

分 类 号：S828.2[农业科学—畜牧学]