不同浓度丁香酚对河川沙塘鳢抗氧化酶活性和组织显微结构的影响  

Effects of Different Concentrations of Eugenol on Antioxidant Enzyme Activity and Tissue Microstructure of Odontobutis potamophila

作　　者：刘国兴[1,2] 李杰 周梓涵 郑友 郝忱[1,2] 丁淑燕 蒋琦辰[1,2] LIU Guo-xing;LI Jie;ZHOU Zi-han;ZHENG You;HAO Chen;DING Shu-yan;JIANG Qi-chen(Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China;Low-temperature Germplasm Bank of Important Economic Fish of Jiangsu Provincial Science and Technology Resources(Agricultural Germplasm Resources)Coordination Service Platform,Nanjing 210017,China)

机构地区：[1]江苏省淡水水产研究所,江苏南京210017 [2]江苏省科技资源(农业种质)统筹服务平台重要经济鱼类低温种质库,江苏南京210017

出　　处：《江西农业学报》2025年第4期89-94,111,共7页Acta Agriculturae Jiangxi

基　　金：江苏现代农业产业技术体系建设项目(JATS〔2023〕376、JATS〔2023〕378)。

摘　　要：为提高河川沙塘鳢(Odontobutis potamophila)在常温下的养殖生产操作及运输中的存活率,设置6、8、10和12 mg/L等4个丁香酚浓度梯度组对河川沙塘鳢进行了麻醉试验,在试验进行8 h后观察河川沙塘鳢肝脏组织和鳃组织的变化,并测定肝脏组织和鳃组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)含量的变化。结果表明,8 h后各试验组的河川沙塘鳢经历了完整的麻醉和复苏过程,仍全部存活;其肝脏组织和鳃组织结构未出现明显的组织损伤。8 h后各试验组在河川沙塘鳢肝脏和鳃组织中的SOD、CAT和GSH-PX活性显著高于对照组(P<0.05),而MDA含量与对照组无显著差异。试验证明了在河川沙塘鳢的运输中,丁香酚会刺激鱼体产生氧化应激反应,但对鱼体细胞不会产生损伤,因此,适宜质量浓度的丁香酚可作为河川沙塘鳢运输中的麻醉剂。To enhance the survival rate of Odontobutis potamophila during breeding,production,operation and transportation at ambient temperature,this study conducted an anesthesia experiment using eugenol on Odontobutis potamophila specimens,and the mass concentrations of eugenol were established at 6,8,10 and 12 mg/L.Following an 8-hour experimental period,alterations in the liver and gill tissues of Odontobutis potamophila were examined.Additionally,the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-PX),along with variations in malondialdehyde(MDA)content,were assessed in both liver and gill tissues.The results showed that following an 8-hour period,Odontobutis potamophila in each experimental group experienced a full anesthesia and recovery cycle,with a 100%survival rate.Histological examination revealed no significant damage to the liver or gill tissue structures.Following an 8-hour testing period,the activities of superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-PX)in the liver and gill tissues were significantly elevated compared to the control group(P<0.05).In contrast,the malondialdehyde(MDA)content did not differ significantly from the control group.Empirical studies have demonstrated that the application of eugenol during the transportation of Odontobutis potamophila induces an oxidative stress response within the body,without inflicting cellular damage.Consequently,eugenol,when administered at an optimal concentration,can serve as an effective anesthetic for Odontobutis potamophila during the transportation.

关 键 词：河川沙塘鳢 丁香酚 组织结构 抗氧化酶 麻醉 

分 类 号：Q959.481[生物学—动物学]