铜胁迫对‘中山杉406’生理特性的影响  

Effects of Copper Stress on the Physiological Characteristics of Taxodium hybrid‘Zhongshanshan 406’

作　　者：胡加玲 於朝广[2] 徐裕玲 梁文超 谢寅峰[3] Hu Jialing;Yu Chaoguang;Xu Yuling;Liang Wenchao;Xie Yinfeng(Co-Innovation Center for Sustainable Forestry in Southern China(Nanjing Forestry University),Nanjing 210037,P.R.China;Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Zhongshan Botanical Garden));Co-Innovation Center for Sustainable Forestry in Southern China(Nanjing Forestry University))

机构地区：[1]南方现代协同创新中心(南京林业大学),南京210037 [2]江苏省植物资源研究与利用重点实验室(江苏省中国科学院植物研究所(南京中山植物园)) [3]南方现代协同创新中心(南京林业大学)

出　　处：《东北林业大学学报》2025年第6期25-34,共10页Journal of Northeast Forestry University

基　　金：江苏省林业科技创新与推广项目(LYKJ[2021]05)。

摘　　要：以‘中山杉406’(Taxodium hybrid‘Zhongshanshan 406’)及其亲本落羽杉[Taxodium distichum(Linn.)Rich.]1年生扦插苗为供试苗木,于4月初将苗木移至塑料盆中,用清水培养2周后换至0.5倍质量浓度的霍格兰(Hoagland)营养液中进行培养,用于铜胁迫处理;以硫酸铜(CuSO_(4))为铜源,设置铜(Cu^(2+))浓度分别为0.2、0.3、0.4 mmol/L,以不施加铜处理液为对照,采用水培法胁迫处理28 d;按照试验设计,测定生物量、植株相对含水量、叶片相对含水量、叶绿素质量分数、丙二醛质量摩尔浓度、细胞膜透性、超氧化物歧化酶活性、过氧化物酶活性、PSⅡ最大光化学效率(F_(v)/F_(m))、PSⅡ有效光化学效率(F_(v)′/F_(m)′)、PSⅡ实际光化学效率(φ(PSⅡ))、光化学淬灭系数(C(PQ))、非光化学淬灭系数(C(NPQ));采用相关性分析、灰色关联度分析方法,分析不同浓度铜胁迫对‘中山杉406’及其亲本落羽杉1年生扦插苗生长、抗氧化系统及叶绿素荧光参数的影响。结果表明:处理28 d期间,随着铜胁迫浓度的增加,‘中山杉406’和落羽杉幼苗的生物量、含水量和叶片叶绿素质量分数均呈下降趋势,而细胞质膜透性和丙二醛质量摩尔浓度则呈上升趋势。与落羽杉相比,铜胁迫后‘中山杉406’的上述指标变化幅度较小。铜浓度为0.3、0.4 mmol/L处理对‘中山杉406’和落羽杉生物量均影响显著。随着胁迫时间和强度的增加,‘中山杉406’与落羽杉的超氧化物歧化酶活性总体均呈先上升后下降的趋势,‘中山杉406’维持峰值时间较长(至处理21 d),而落羽杉在处理14 d后即出现明显下降。‘中山杉406’的过氧化物酶活性呈持续增加趋势,落羽杉在21 d基本达到峰值。表明‘中山杉406’的超氧化物歧化酶、过氧化物酶,在胁迫后期能维持相对较高的活性。两种植物的叶绿素荧光参数,PSⅡ最大光化学效率、PSⅡ有效光化学效率、PSⅡ实际光One-year-old cuttings of Taxodium hybrid‘Zhongshanshan 406’and its parent[Taxodium distichum(Linn.)Rich.]were used as test seedlings.In early April,the seedlings were transplanted into plastic pots and cultured in clean water for 2 weeks,then transferred to 0.5-strength Hoagland nutrient solution for copper stress treatment.Copper sulfate(CuSO_(4))was used as the copper source,and copper(Cu^(2+))concentrations were set at 0.2,0.3,and 0.4 mmol/L respectively,with the treatment without copper added as the control.The hydroponic method was used for stress treatment for 28 days.According to the experimental design,biomass,relative water content of the plant,relative water content of leaves,chlorophyll content,malondialdehyde molar concentration,cell membrane permeability,superoxide dismutase activity,peroxidase activity,maximum photochemical efficiency of PSII(F_(v)/F_(m)),effective photochemical efficiency of PSII(F_(v)′/F_(m)′),actual photochemical efficiency of PSII(φ(PSⅡ)),photochemical quenching coefficient(C(PQ)),and non-photochemical quenching coefficient(C(NPQ))were measured.Correlation analysis and grey relational analysis were used to analyze the effects of different concentrations of copper stress on the growth,antioxidant system,and chlorophyll fluorescence parameters of one-year-old cuttings of T.‘Zhongshanshan 406’and its parent T.distichum.The results showed that during the 28-day treatment period,with the increase of copper stress concentration,the biomass,water content,and leaf chlorophyll content of T.‘Zhongshanshan 406’and T.distichum seedlings all showed a downward trend,while the cytoplasmic membrane permeability and malondialdehyde molar concentration showed an upward trend.Compared with T.distichum,the changes in the above-mentioned indicators of T.‘Zhongshanshan 406’after copper stress were smaller.Treatments with copper concentrations of 0.3 and 0.4 mmol/L had significant effects on the biomass of both T.‘Zhongshanshan 406’and T.distichum.With the increase of stress

关 键 词：‘中山杉406’ 生理特性 铜胁迫 抗氧化酶活性 叶绿素荧光参数 

分 类 号：S718.43[农业科学—林学]