不同遗传型土生空团菌对马尾松生长影响及促生效果评价  

The Influence of Different Genotypes of Cenococcum geophilum on the Growth of Pinus massoniana Seedlings and the Evaluation of Their Growth-promoting Effects

作　　者：张玉 虎闫添艺 张骁 钟诗焕 张桃香 Zhang Yuhu;Yan Tianyi;Zhang Xiao;Zhong Shihuan;Zhang Taoxiang(Fujian Agriculture and Forestry University,Fuzhou 350000,P.R.China;Institute of Subtropical Agroecology,Chinese Academy of Sciences;Fujian Agriculture and Forestry University)

机构地区：[1]福建农林大学,福州350000 [2]中国科学院亚热带农业生态研究所 [3]福建农林大学

出　　处：《东北林业大学学报》2025年第6期88-97,共10页Journal of Northeast Forestry University

基　　金：福建省自然科学基金项目(2021J01061)。

摘　　要：以土生空团菌(Cenococcum geophilum)和马尾松(Pinus massoniana)幼苗为研究对象,选用9种不同遗传型土生空团菌(C_(1)~C_(9))在温室中进行盆栽接种试验,以未接种处理为对照,共计10个处理;接种处理的马尾松幼苗生长3个月后,测定其生长指标、土壤理化性质、土壤酶活性;采用单因素方差分析法(ANOVA)、主成分分析法、隶属函数法,分析不同遗传型土生空团菌对马尾松幼苗促生效果。结果表明:土生空团菌促进了马尾松幼苗的生长,其中C_(6)、C_(7)、C_(8)菌株对马尾松幼苗的根系结构、光合作用和根际土壤中脲酶和酸性磷酸酶的活性等具有显著促进作用(P<0.05)。菌株对马尾松幼苗促生效果,由高到低依次为C_(6)、C_(8)、C_(5)、C_(9)、C_(7)、C_(4)、C_(2)、C_(1)、C_(3);通过聚类分析将其划分为4个不同等级,分别为平稳型(C_(3)菌株)、低促型(C_(4)、C_(2)、C_(1)菌株)、中促型(C_(8)、C_(5)、C_(9)、C_(7)菌株)、高促型(C_(6)菌株)。土生空团菌促进马尾松苗木生长,其中根体积、全钾质量分数、酸性磷酸酶(ACP)活性、蒸腾速率、脲酶(UE)活性、根总长,为土生空团菌对马尾松幼苗促生效果评价的主要指标;C_(6)、C_(8)、C_(5)、C_(9)、C_(7)菌株对马尾松幼苗促生效果较好。Using Cenococcum geophilum and Pinus massoniana seedlings as research subjects, a pot inoculation experiment was conducted in a greenhouse with nine different genotypes of Cenococcum geophilum (C_(1) to C_(9)), with a non-inoculated treatment as the control, totaling 10 treatments. After three months of growth, the growth parameters, soil physicochemical properties, and soil enzyme activities of the inoculated P. massoniana seedlings were measured. One-way analysis of variance (ANOVA), principal component analysis (PCA), and membership function analysis were used to evaluate the growth-promoting effects of different genotypes of C. geophilum on P. massoniana seedlings. The results showed that C. geophilum promoted the growth of P. massoniana seedlings, with strains C_(6), C_(7), and C_(8) significantly enhancing root architecture, photosynthesis, and the activities of urease and acid phosphatase in the rhizosphere soil ( P <0.05). The growth-promoting effects of the strains on P. massoniana seedlings, ranked from high to low, were as follows: C_(6), C_(8), C_(5), C_(9), C_(7), C_(4), C_(2), C_(1), and C_(3). Cluster analysis classified them into four levels: stable type (C_(3)), low-promoting type (C_(4), C_(2), C_(1)), medium-promoting type (C_(8), C_(5), C_(9), C_(7)), and high-promoting type (C_(6)). C. geophilum promoted the growth of P. massoniana seedlings, with root volume, total potassium content, acid phosphatase (ACP) activity, transpiration rate, urease (UE) activity, and total root length identified as the main indicators for evaluating the growth-promoting effects. Strains C_(6), C_(8), C_(5), C_(9), and C_(7) showed better growth-promoting effects on P. massoniana seedlings.

关 键 词：土生空团菌 马尾松 生长指标 土壤理化性质 

分 类 号：S718.8[农业科学—林学] S714.3