多巴胺改性丝胶蛋白粘合剂的制备及性能  

Preparation and properties of dopamine-modified sericin adhesive

作　　者：李奕潇 李智鹏 吴子微 王昊宇 卢神州[1,2] 邢铁玲 LI Yixiao;LI Zhipeng;WU Ziwei;WANG Haoyu;LU Shenzhou;XING Tieling(College of Textile and Clothing Engineering,Soochow University,Suzhou Jiangsu 215021,China;Shengzhou Institute of Silk Fibroin Biotechnology Applications,Shengzhou Zhejiang 312400,China)

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215021 [2]嵊州市丝素蛋白生物技术应用研究院,浙江嵊州312400

出　　处：《纺织工程学报》2025年第2期54-64,共11页JOURNAL OF ADVANCED TEXTILE ENGINEERING

基　　金：国家自然科学基金资助项目(51973144);江苏高等学校优势学科建设项目(PAPD)。

摘　　要：开发具有强大水下粘附性并具有抗菌性能的粘合剂是一个挑战。通过多巴胺(DA)氧化自聚合对丝胶蛋白分子链进行湿粘附改性,并通过单宁酸(TA)对改性的蛋白分子链进行交联,制备了TA-DA/Ser粘合剂。测试结果表明:粘合剂在宏观上表现出粘弹性固态,能够提供较好的机械强度。在溶液中对丝胶蛋白成功改性,且氢键是单宁酸与改性丝胶蛋白交联的主要驱动力。粘合剂对猪皮组织的粘附强度最高达到86.22 kPa,对1,1-二苯基-2-三硝基苯肼(DPPH)的清除率高达91.62%,对大肠杆菌和金黄色葡萄球菌的抑菌率分别为98.93%和98.17%。细胞毒性和伤口愈合实验表明,粘合剂达到细胞毒性二级标准,且对SD大鼠的伤口愈合起到促进作用。TA-DA/Ser粘合剂在无缝合伤口闭合和组织粘附中具有应用潜力。Developing adhesives with strong underwater adhesion and antibacterial properties is a significant challenge.Sericin molecular chains were modified for wet adhesion via dopamine(DA)oxidative self-polymerization,and the modified protein chains were cross-linked with tannic acid(TA)to prepare a TA-DA/Ser adhesive.Test results indicated that the adhesive exhibited viscoelastic solid behavior at the macroscopic level,providing good mechanical strength.The successful modification of silk fibroin in solution was confirmed,and hydrogen bonding was identified as the primary driving force for the cross-linking between tannic acid and the modified sericin.The adhesive demonstrated a maximum adhesion strength of 86.22 kPa on pigskin tissue,a DPPH radical scavenging rate of up to 91.62%,and survival rates of only 1.07%and 1.03%for Escherichia coli and Staphylococcus aureus,respectively.Cytotoxicity and wound healing experiments showed that the adhesive met the Grade II cytotoxicity standard and promoted wound healing in SD rats.The TA-DA/Ser adhesive holds potential applications in suture-free wound closure and tissue adhesion.

关 键 词：多巴胺 丝胶蛋白 单宁酸 水下粘合剂 抗菌性能 

分 类 号：Q816[生物学—生物工程]