SPCSV和SPFMV协生互作对甘薯生理及细胞病理学变化的影响  

Effects of synergism between SPCSV and SPFMV on physiological and cytopathological changes of sweet potato

作　　者：李燃 田雨婷[2] 赵付枚 李春奇[1] 张振臣 LI Ran;TIAN Yuting;ZHAO Fumei;LI Chunqi;ZHANG Zhenchen(College of Life Sciences,Henan Agricultural University,Zhengzhou 450046,China;Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南农业大学生命科学学院,郑州450046 [2]河南省农业科学院植物保护研究所,郑州450002

出　　处：《植物病理学报》2025年第2期203-211,共9页Acta Phytopathologica Sinica

基　　金：河南省优势学科培育联合基金重点项目(222301420029);国家甘薯产业技术体系建设项目(CARS-10-B15)。

摘　　要：以分别单独感染甘薯褪绿矮化病毒(sweet potato chlorotic stunt virus,SPCSV)和甘薯羽状斑驳病毒(sweet potato feathery mottle virus,SPFMV)以及复合感染两种病毒的甘薯植株为材料,研究了SPCSV和SPFMV协生对甘薯光合作用等生理特征以及叶片表皮亚显微结构、叶绿体超微结构等细胞病理学变化的影响。结果表明,SPCSV和SPFMV复合侵染显著降低了净光合速率(A)、气孔导度(Gs)、蒸腾速率(E)、CO_(2)利用效率(CUE)和叶绿素(ChI)含量;提高了甘薯叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性及丙二醛(MDA)含量。扫描电镜观察结果显示,与健康对照相比,SPFMV和SPCSV单侵染时甘薯叶片表皮细胞和气孔无明显变化,而两种病毒的复合侵染可引起甘薯叶片外表皮气孔关闭,叶片表面凹陷,保卫细胞萎缩。透射电镜观察发现,与健康对照相比,SPFMV和SPCSV单独侵染时叶绿体数量没有明显变化,但对其超微结构有一定影响,包括淀粉粒增多,体积膨大、部分基质和基粒片层排列疏松;当SPCSV和SPFMV复合侵染则可导致叶绿体数量明显减少,叶绿体内部结构和膜遭到严重破坏,细胞质中发现大量风轮状内含体。The effects of synergism between sweet potato chlorotic stunt virus(SPCSV)and sweet potato feathery mottle virus(SPFMV)on photosynthesis,submicroscopic leaf epidermis structure,and chloroplast ultrastructure of sweet potato were studied using sweet potato plants infected with either SPCSV,SPFMV,or a combination of the two.The results showed that the net photosynthetic rate,stomatal conductance,transpiration rate,CO_(2) utilization efficiency,and chlorophyll content of sweet potato plants were significantly decreased,and the activities of antioxidant enzymes and malondialdehyde content were increased due to the co-infection of SPCSV and SPFMV.Scanning electron microscopy showed that the co-infection of SPCSV and SPFMV could induce stomatal closure,leaf surface depression,and guard cell shrinkage,whereas no significant changes occurred in the single-infected plants,compared to the healthy plants.By using a transmission electron microscope,we found that the co-infection with SPCSV and SPFMV resulted in a reduction in the number and severe distortion of chloroplasts and the presence of large numbers of cylindrical inclusions in the cytoplasm of sweet potato plants.In the single-infected sweet potato plants,the number of chloroplasts remained unchanged,while some alternations on the ultrastructure were found,including increasedswollen starch granules and some loosely arranged grana and stroma lamellae.

关 键 词：甘薯羽状斑驳病毒 甘薯褪绿矮化病毒 病毒协生 生理特征 细胞病理学 

分 类 号：S432.1[农业科学—植物病理学]