灰葡萄孢分泌蛋白BcXYG3的功能研究  

Functional characterization of a secretory protein BcXYG3 in Botrytis cinerea

作　　者：聂晓飞 王紫瑶 毕凯 朱闻君 NIE Xiaofei;WANG Ziyao;BI Kai;ZHU Wenjun(School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学生命科学与技术学院,武汉430023

出　　处：《植物病理学报》2025年第2期212-224,共13页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31972215)。

摘　　要：灰葡萄孢(Botrytis cinerea)是一种寄主范围广泛的死体营养型植物病原真菌,可在多种重要农作物上引发灰霉病。灰葡萄孢通过分泌大量的细胞死亡诱导蛋白(CDIPs)诱发寄主细胞死亡,从而促进其侵染。本研究通过对灰葡萄孢侵染阶段的分泌蛋白组进行分析,筛选到一个分泌蛋白BcXYG3。BcXYG3含有GH12和fCBD结构域,在本氏烟草叶片中瞬时表达BcXYG3可诱导叶片细胞死亡,而瞬时表达去掉信号肽的BcXYG3则无法诱导叶片坏死,推测该蛋白可能是被分泌到植物质外体空间发挥作用。BcXYG3基因在灰葡萄孢侵染阶段上调表达,但将其敲除或过量表达后,病菌的致病力、生长速度、产孢量和抗逆性无明显变化。此外,在本氏烟草叶片中注射纯化的BcXYG3能诱导植物对灰葡萄孢的抗性。综上所述,灰葡萄孢分泌蛋白BcXYG3可引起植物细胞坏死并参与诱导植物产生抗病性,在灰葡萄孢与植物互作中起着重要作用。研究结果为进一步阐明灰葡萄孢-寄主植物互作机理,以及为灰霉病害的有效防治提供理论依据和基因资源。Botrytis cinerea,a phytopathogenic fungi with a wide host range,can cause gray mold disease in many important crops.During infection of plant,B.cinerea secretes numerous cell death-inducing proteins(CDIPs)to induce host cell death,which as a result promotes its infection.In this study,we analyzed the secreted proteome during the infection stage of B.cinerea and identified a secreted protein BcXYG3,which contains GH12 and fCBD domains.Transiently expression of BcXYG3 rather than BcXYG3Δsp(BcXYG3 without a signal peptide)in Nicotiana benthamiana leaves could induce cell death,suggesting that BcXYG3 possibly functions in plant cell apoplastic space.The expression of BcXYG3 was upregulated during the infection stage of B.cinerea.However,deletion or over-expression of BcXYG3 did not significantly affect the pathogenicity,growth rate,conidial production,and some stress tolerance of the pathogen.In addition,infiltration of purified BcXYG3 into N.benthamiana leaves could trigger plant resistance and the expression of defense-related genes.In conclusion,the secreted protein BcXYG3 of B.cinerea can trigger cell necrosis and resistance in plant,playing significant roles in the interaction between B.cinerea and host plant.The result is helpful for clarifying the mechanism underlying B.cinerea-plant interaction and provides theoretical basis and genetic resource for breeding of resistant crop varieties against gray mold disease.

关 键 词：灰葡萄孢 CDIPs BcXYG3 灰葡萄孢-植物互作 

分 类 号：S432.44[农业科学—植物病理学]