桃褐腐病菌自噬相关基因MfATG1生物学功能研究  

Study on the biological functions of the autophagy related gene MfATG1 in Monilinia fructicola

作　　者：黄松 曾哲政 尹良芬 阴伟晓 罗朝喜[1,2] HUANG Song;ZENG Zhezheng;YIN Liangfen;YIN Weixiao;LUO Chaoxi(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,Huazhong Agricultural University,Wuhan 430070,China;Hubei Key Lab of Plant Pathology and College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学果蔬园艺作物种质创新与利用全国重点实验室,武汉430070 [2]华中农业大学植物科技学院湖北省作物病害监测及安全控制重点实验室,武汉430070

出　　处：《植物病理学报》2025年第2期225-236,共12页Acta Phytopathologica Sinica

基　　金：国家桃产业技术体系(CARS-30),国家自然科学基金(31371896)。

摘　　要：桃褐腐病是桃树主要病害之一,其大规模流行给果农带来巨大损失。目前,关于褐腐病的研究主要集中在病原菌的分离鉴定、抗药性、防治等方面,而对褐腐病菌致病机制的研究相对较少。本研究基于前期获得的桃褐腐病菌(Monilinia fructicola)侵染果实的早期转录组数据及基因组数据,通过PHI注释分析发现自噬相关基因MfATG1在M.fructicola侵染过程中可能发挥重要作用。通过基因敲除技术,获得MfATG1基因的敲低阳性转化子,并比较其与野生型菌株生长发育、致病力和其他抗逆性的差异,以解析其生物学功能。研究结果表明:MfATG1基因敲低影响M.fructicola菌丝形态和自噬过程,降低菌丝生长速率和致病力,但不影响孢子萌发。在NaCl胁迫条件下,MfATG1敲低提高了M.fructicola对NaCl的敏感性,表明MfATG1基因参与M.fructicola对盐胁迫的应答。在H_(2)O_(2)、SDS和刚果红胁迫条件下,敲低MfATG1降低了M.fructicola对其敏感性,表明MfATG1基因参与调控M.fructicola的细胞壁完整性以及氧化应激反应。Peach brown rot is one of the major diseases of peach,which causes significant losses to growers when it occurs in large scale.Currently,research on brown rot disease mainly focuses on the isolation and identification of the pathogen,fungicides resistance,and control measures.While the study on the pathogenic mechanism of the causing agent Monilinia fructicola,is relatively rare.This study is based on the previously obtained genome of M.fructicola and its early-stage transcriptome from infected fruit.Through PHI annotation analysis,it was found that the MfATG1 gene may play an important role in the infection process of M.fructicola.By using gene knockout technology,the knockdown positive transformants of MfATG1 gene were obtained,and their growth and development,virulence,and other stress-resistant phenotypes were compared with the wild-type strain to analyze the biological functions of the gene.The research results showed that the knockdown of MfATG1 gene affected the mycelial morphology and autophagy process,reduced mycelial growth rate and virulence of M.fructicola,but does not affect spore germination,indicating that MfATG1 gene is involved in the regulation of mycelial growth,virulence,and autophagy process in M.fructicola.Under NaCl stress conditions,the knockdown transformants increased the sensitivity to NaCl,indicating that the MfATG1 gene is involved in the response to salt stress.Under H_(2)O_(2),SDS,and Congo red stress conditions,the knockdown transformants reduced the sensitivity to them,indicating that MfATG1 gene participates in the control of cell wall integrity and the oxidative stress response in M.fructicola.

关 键 词：桃褐腐病菌 MfATG1基因 致病力 自噬 逆境胁迫 

分 类 号：S432.44[农业科学—植物病理学]