高粱NF-Y转录因子序列特征、表达及生物胁迫响应  

Sequence characteristics,expression and response to biological stress of NF-Y transcription factors in sorghum(Sorghum bicolor)

作　　者：杨博慧 粱月秀 柴文婷 赵珊珊 冯凡 朱立勋 范佳利 邹春雷 郝艳平 赵威军 吕晋慧 孙文献 张春来[1] YANG Bohui;LIANG Yuexiu;CHAI Wenting;ZHAO Shanshan;FENG Fan;ZHU Lixun;FAN Jiali;ZOU Chunlei;HAO Yanping;ZHAO Weijun;LÜJinhui;SUN Wenxian;ZHANG Chunlai(College of Agronomy,Shanxi Agricultural University,Ministry of Education and Shanxi Province Cofunded Collaboration and Innovation Center for Speciality Crops on the Loess Plateau,Taigu 030801,China;Sorghum Research Institute,Shanxi Agricultural University,Taigu 030801,China;Plant Protection College,China Agricultural University,Beijing 100193,China)

机构地区：[1]山西农业大学农学院,教育部省部共建黄土高原特色作物协同创新中心,太谷030801 [2]山西农业大学高粱研究所,太谷030600 [3]中国农业大学植物保护学院,北京100193

出　　处：《植物病理学报》2025年第2期237-250,共14页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31971994、31470285);山西农科院博士基金(ybsj1404);山西省自然基金重点项目(2014011004);山西省百人计划合作共建项目(晋组2017-30)。

摘　　要：核转录因子Y(nuclear transcription factor Y,NF-Y)在植物生长发育和非生物胁迫应答中起着重要的作用,但对其参与昆虫、微生物互作的机制研究较少。本研究旨在鉴定高粱NF-Y家族的基因成员,分析其序列特征,测定在高粱组织基因表达时空差异及响应高粱蚜(Melanaphis sacchari)侵染和丝轴黑粉菌(Sporisorium reilianum)侵染,并分析了自然等位DNA变异和蛋白互作网络。在高粱基因组筛选到9个NF-YA、15个NF-YB和12个NF-YC亚家族成员,在10条染色体上不均匀分布,同一亚家族中的成员基因结构相似,且大多数亲缘关系较近的SbNF-Ys的外显子-内含子结构较为相似。蛋白结构域分析结果显示,CBFB_NFYA为NF-YA亚家族的保守结构域,CBFD_NFYB_HMF为NF-YB和NF-YC亚家族的保守结构域;对基因启动子顺式作用元件分析发现,SbNF-Ys的启动子区域含有大量核心元件,如光响应元件和多种响应环境和激素的元件;系统进化树分析表明,SbNF-Ys在进化过程上较为保守,单子叶和双子叶植物间不存在明显分化,高粱与玉米的NF-Ys关系最近;基因表达分析表明,高粱NF-Y家族成员在不同高粱品种和组织中的表达量存在显著差异。高粱蚜侵染后抗、感蚜品系SbNF-YB3.2g、SbNF-YB11.7g的表达水平显著升高;感丝黑穗病品系较抗病品系SbNF-YB10.2g和SbNF-YB11.7g的表达水平显著升高。检测到SNP/INDEL DNA变异可建立分子辅助育种标记。本研究初步分析了高粱NF-Y家族的基因结构与表达,旨在为高粱抗蚜虫、抗丝黑穗病育种提供理论基础。The nuclear transcription factor Y(NF-Y)plays an important role in plant growth and development and in the response to abiotic stresses,but the mechanisms by which they are involved in insect and microbial interactions are less well understood.The purpose of this study was to identify the gene members of the NF-Y family of sorghum by bioinformatics,analyze their sequence characteristics,determine the spatio-temporal differences in gene expression in sorghum tissues,respond to Melanaphis sacchari and Sporisorium reilianum infection and DNA variation analysis.In the sorghum genome screened to 9 NF-YA subfamilies,15 NF-YB subfamilies and 12 NF-YC subfamilies,is unevenly distributed on 10 chromosomes,and the gene structure of members in the same subfamily is similar,and most of the closely related members of the SbNF-Y family have similar exon-intron structures and conservative motifs.The encoded NF-Y proteins are all hydrophilic proteins,and the secondary structure is dominated byαhelix and random curl;The results of protein domain analysis showed that the CBFB_NFYA was a conserved domain of the NF-YA subfamily,CBFD_NFYB_HMF a conserved domain of the NF-YB and NF-YC subfamilies.Analysis of gene promoter cis-acting elements showed that the promoter region of the NF-Y family of sorghum contained a large number of core elements,such as photoresponsive elements and a variety of elements that responded to the environment and hormones.Phylogenetic tree analysis showed that the NF-Y family was relatively conserved in the evolutionary process,and there was no obvious differentiation between monocotyledons and dicotyledons,and the relationship between sorghum NF-Y and maize was the closest;Gene expression analysis showed that there were significant differences in the expression of members of the NF-Y family of sorghum in different sorghum varieties and tissues.Expression of SbNF-YB3.2g and SbNF-YB11.7g were significantly enhanced after cane aphid infestation.Expression of SbNF-YB10.2g and SbNF-YB11.7g were significantly enh

关 键 词：高粱 NF-Y 高粱蚜 丝黑穗病 自然等位DNA变异 

分 类 号：S514[农业科学—作物学]