柑橘褐斑病菌的种群构成及优势种-盖森链格孢对两种SDHIs杀菌剂的敏感基线  

Population composition of the pathogens causing citrus brown spot and baseline sensitivity of the dominant pathogenic species-Alternaria gaisen to boscalid and fluopyram

作　　者：叶璐 张超凡 程苗嘉 王文 马慧 王璟睿 李红叶[1] 焦晨 YE Lu;ZHANG Chaofan;CHENG Miaojia;WANG Wen;MA Hui;WANG Jingrui;LI Hongye;JIAO Chen(Laboratory of Molecular Biology of Crop Pathogens and Insects/Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects/College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]农业农村部作物病虫分子生物学重点实验室/浙江省作物病虫生物学重点实验室/浙江大学农业与生物技术学院,杭州310058

出　　处：《植物病理学报》2025年第2期281-288,共8页Acta Phytopathologica Sinica

基　　金：国家自然科学基金项目(32102148,32072362);国家现代农业(柑橘)产业技术体系建设专项(CARS-26)。

摘　　要：为了明确中国柑橘褐斑病菌的种群组成及优势种对琥珀酸脱氢酶抑制剂(SDHIs)的敏感性,本研究针对7个柑橘主产区的135个柑橘褐斑病样品进行了病原分离鉴定和优势种群的药剂敏感性测试。在本次分离获得的155个链格孢属(Alternaria)菌株中,盖森链格孢(A.gaisen)的数量已超越交链格孢(A.alternata),成为新的优势病原种群。采用菌丝生长速率法测定了100个盖森链格孢菌株对啶酰菌胺和氟吡菌酰胺的敏感性,结果表明:啶酰菌胺对供试菌株的EC_(50)值范围为0.4129~4.7137 mg·L^(-1),平均值为1.9257 mg·L^(-1);氟吡菌酰胺对供试菌株的EC_(50)值范围为0.1272~3.3648 mg·L^(-1),平均值为1.1149 mg·L^(-1)。盖森链格孢群体对这两种药剂的敏感性分布均为连续的单峰曲线,且通过K-S检验(Kolmogorov-Smirnov test)验证符合正态分布,未发现抗药性菌株。因此,1.9257 mg·L^(-1)和1.1149 mg·L^(-1)可分别被确定为盖森链格孢对啶酰菌胺和氟吡菌酰胺的敏感性基线。此外,来自不同地区的盖森链格孢对这两种药剂的敏感性差异不显著(P>0.05),所有供试菌株对氟吡菌酰胺的敏感性普遍高于啶酰菌胺。本研究为利用啶酰菌胺和氟吡菌酰胺防治柑橘褐斑病提供了重要的数据支持和技术依据。To clarify the population composition of pathogens causing citrus brown spot in China and their sensitivity to succinate dehydrogenase inhibitors(SDHIs),135 diseased samples from 7 major citrus-producing regions were collected and pathogen isolation and identification was conducted in this study.Meanwhile,fungicide sensitivity test of the dominant pathogen species was carried out.Among 155 Alternaria strains isolated,the number of A.gaisen has surpassed that of A.alternata,becoming the new dominant pathogenic population.The sensitivity of 100 A.gaisen strains to boscalid and fluopyram was determined using the mycelial growth rate method.The results showed that the EC_(50) values for boscalid ranged from 0.4129 to 4.7137 mg·L^(-1),with an average value of 1.9257 mg·L^(-1);for fluopyram,the EC_(50) values ranged from 0.1272 to 3.3648 mg·L^(-1),with an average value of 1.1149 mg·L^(-1).The sensitivity curves of the A.gaisen population to these two fungicides were continuous and unimodal,conforming to a normal distribution according to the Kolmogorov-Smirnov test(K-S test),and no strains showed resistance.Therefore,1.9257 mg·L^(-1) and 1.1149 mg·L^(-1) were established as the sensitivity baselines for A.gaisen to boscalid and fluopyram,respectively.Moreover,there were no significant differences(P>0.05)in sensitivity of A.gaisen from different regions to these two fungicides,with all strains showing generally higher sensitivity to fluopyram than to boscalid.This study provides important data support and technical basis for the utilization of boscalid and fluopyram in the control of citrus brown spot disease.

关 键 词：柑橘褐斑病 盖森链格孢 交链格孢 啶酰菌胺 氟吡菌酰胺 敏感基线 

分 类 号：S436.661.1[农业科学—农业昆虫与害虫防治]