贝莱斯芽孢杆菌挥发性有机化合物对番茄灰葡萄孢的抑制及活性组分分析  

Antagonistic activity and active components of volatile organic compounds(VOCs)from Bacillus velezensis C11 against Botrytis cinerea causing tomato gray mold

作　　者：邓安玲 常若葵 李卓然 于玮玮 苑瑜瑾 王远宏 DENG Anling;CHANG Ruokui;LI Zhuoran;YU Weiwei;YUAN Yujin;WANG Yuanhong(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300384,China;College of Engineering and Technology,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]天津农学院工程技术学院,天津300384

出　　处：《植物病理学报》2025年第2期301-312,共12页Acta Phytopathologica Sinica

基　　金：甘肃省科技项目计划(22CX8NH226);甘肃省科技项目计划(23CXNH0014)。

摘　　要：为确定贝莱斯芽孢杆菌(Bacillus velezensis)C11菌株产生的挥发性有机化合物(volatile organic compounds,VOCs)对番茄灰葡萄孢(Botrytis cinerea)的生防效果,采用双皿对扣法测定VOCs对番茄灰葡萄孢菌丝生长、分生孢子形成、细胞膜通透性、菌丝显微形态的影响,对番茄灰霉病的防治效果,以及对番茄体内抗性相关的POD、CAT酶活的影响。结果表明,B.velezensis C11 VOCs能显著抑制番茄灰葡萄孢菌丝生长,抑制率达78.35%;可造成番茄灰葡萄孢产孢量下降、细胞膜受损、菌丝形态改变等;对番茄灰霉病的防效达76.9%。进一步通过SPME-GC-MS检测B.velezensis C11 VOCs成分,共鉴定出27种物质,其中8种(2-庚酮、2-壬酮、6甲基-2-庚酮、2-甲氧基酚、2-乙基-5甲基吡嗪、2-十一醇、2-十三烷酮、2-十一酮)相对含量较高(占总含量5.43%)。当浓度为0.5 nmol·mL^(-1)时,这8种物质对番茄灰葡萄孢均有较好的抑菌活性。本研究明确了B.velezensis C11菌株可通过产生具有抑菌活性的VOCs发挥生防作用,同时初步分析鉴定了其VOCs中主要的活性抑菌组分,为深入解析该菌株的生防机制以及将其应用于生产实践提供了理论依据。To determine the biocontrol effect of volatile organic compounds(VOCs)produced by Bacillus velezensis C11 strain against Botrytis cinerea,the pathogen causing tomato gray mold,a double petri dish assay was performed to evaluate the effect of B.velezensis C11 VOCs on mycelial growth,micromorphology,sporulation and cell membrane permeability of B.cinerea,on the occurrence and severity of gray tomato through detached leaf inoculation test,and on the activity of defense-related enzymes including POD and CAT in tomato leaves.The results showed that VOCs produced by B.velezensis C11 significantly inhibited mycelial growth of B.cinerea,with an inhibition rate of 78.35%;led to significant decrease in sporulation,cell membrane damage,and change in mycelial micromorphology;had a control effect of 76.9%on tomato gray mold.SPME-GC-MS assay was further employed to analyze the active components of B.velezensis C11 VOCs,and 27 substances were identified,8 of which accounted for a relatively high proportion(5.43%).All these 8 compounds showed significantly inhibitory effect against B.cinerea at a concentration of 0.5 nmol·mL^(-1).In conclusion,B.velezensis C11 strain can inhibit B.cinerea by producing VOCs.This study provides a theoretical basis for further investigation of the antagonistic mechanism of B.velezensis C11 strain.

关 键 词：贝莱斯芽孢杆菌 番茄灰霉病 挥发性有机化合物 生物防治 

分 类 号：S432.1[农业科学—植物病理学]