沼泽红假单胞菌分离鉴定及其次生物质5-ALA对TMV的抑制效果  

Isolation and identification of Rhodopomonas palustris and analysis of the inhibitory effect of its secondary metabolite 5-ALA on TMV

作　　者：黄鹏 史晓斌[2] 黄莉萍 颜健红 罗敏 付威 谢振宇 王培[2] 陈建斌[2] 杜娇 张德咏[2] 李凡[1] 刘勇[2] HUANG Peng;SHI Xiaobin;HUANG Liping;YAN Jianhong;LUO Min;FU Wei;XIE Zhenyu;WANG Pei;CHEN Jianbin;DU Jiao;ZHANG Deyong;LI Fan;LIU Yong(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Loudi Agricultural Technology Promotion Center Plant Protection Station,Loudi 417000,China;Agriculture and Rural Bureau in Shuangfeng County,Shuangfeng 417700,China;Plant Protection and Inspection Station in Yueyang County,Yueyang 414100,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]湖南省农业科学院植物保护研究所,长沙410125 [3]娄底市农技推广中心植保站,娄底417000 [4]双峰县农业农村局,双峰县417700 [5]岳阳县植保植检站,岳阳县414100

出　　处：《植物病理学报》2025年第2期327-337,共11页Acta Phytopathologica Sinica

基　　金：国家重点研发计划(2022YFD1401200);大宗蔬菜产业体系项目(CARS-23-D-02);湖南省青年人才项目(2022RC1149);国家自然科学基金项目(32030088,32272535);湖南省农业科技创新资金项目(2023CX99)。

摘　　要：利用光合细菌分离培养基从云南省玉溪市猕猴桃根际土中分离光合细菌,并测试其对烟草花叶病毒(tobacco mosaic virus,TMV)的抑制效果;同时,对分离到的光合细菌进行次生代谢产物5-氨基乙酰丙酸(5-aminolevulinic acid,5-ALA)产量测定;随后,对分离到的光合细菌菌株及其次生代谢产物5-ALA开展抗TMV的抑制效果测试,并对其抗病毒机理进行初步分析。所分离到的光合细菌菌株YN-1经形态学鉴定、革兰氏染色、碳源、氮源等生理生化测试以及16S rDNA序列分析,鉴定其为沼泽红假单胞菌(Rhodopseudomonas palustris);YN-1的5-ALA产量高达4.37 mg·L^(-1)。分别喷施YN-1发酵原液和次生代谢产物5-ALA后均能降低供试本氏烟上烟草花叶病的发病率,结果显示YN-1发酵原液较清水和5%氨基寡糖素水剂300倍液分别降低69.88%和24.24%;40 mg·L^(-1)5-ALA较清水、5%氨基寡糖素水剂300倍液分别降低74.70%、36.37%。通过对供试本氏烟相关抗病基因复制水平的检测,发现喷施了YN-1发酵原液和5-ALA后,供试本氏烟SA途径抗病基因较对照有显著的上调。结果表明,所分离到的沼泽红假单胞菌YN-1菌株发酵原液及其40 mg·L^(-1)的次生代谢产物5-ALA能有效降低TMV对烟草的侵染。In order to study the control effect of photosynthetic bacteria isolated from kiwi rhizosphere soil samples of Yuxi City,Yunnan province on tobacco mosaic virus(TMV),we measured the yield of the secondary metabolite 5-aminolevulinic acid(5-ALA)from the isolated photosynthetic bacteria,and screened out a strain YN-1 with the highest yield of 5-ALA.Subsequently,the control effect of YN-1 and its secondary metabolite 5-ALA on TMV was tested,and the mechanism was investigated.The strain was identified as Rhodopseudomonas palustris by morphological identification,gram staining,physiological and biochemical tests of carbon and nitrogen sources and 16S rDNA sequence analysis.The 5-ALA yield of this strain is as high as 4.37 mol·L^(-1).The incidence of TMV was decreased by spraying YN-1 fermentation liquid and secondary metabolite 5-ALA respectively.The results showed that the incidence of TMV on plants treated with YN-1fermentation liquid was 69.88%and 24.24%lower than those with water and 5%amino oligosaccharide 300 times treatments,respectively.The incidence of TMV on plants treated with 40 mg/L 5-ALA decreased by 74.70%and 36.37%compared with those with water and 5%amino oligosaccharide treatments,respectively.Through the analysis of plant resistance genes,it was found that the resistance genes in SA pathway were significantly upregulated compared with the control.The results showed that YN-1 fermentation liquid and 40 mg·L^(-1)5-ALA could effectively reduce the infection degree of TMV.

关 键 词：沼泽红假单胞菌 5-ALA 烟草花叶病毒 抑制效果 机理 

分 类 号：S432.44[农业科学—植物病理学]