Phytopythium引起苹果根腐病研究简报  

Brief report of apple root rot caused by Phytopythium spp.

作　　者：莫艳芳 罗强 普特 施竹凤 杨童雨 李燕梅 陈齐斌[2] 杨佩文[1] MO Yanfang;LUO Qiang;PU Te;SHI Zhufeng;YANG Tongyu;LI Yanmei;CHEN Qibin;YANG Peiwen(Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Dali Town Agricultural Comprehensive Service Center in Dali City,Dali 671003,China)

机构地区：[1]云南省农业科学院农业环境与资源研究所,昆明650205 [2]云南农业大学植物保护学院,昆明650201 [3]大理市大理镇农业综合服务中心,大理671003

出　　处：《植物病理学报》2025年第2期338-342,共5页Acta Phytopathologica Sinica

基　　金：云南省科技计划重大科技专项(202202AE090029);国家自然科学基金(32060624)。

摘　　要：随着苹果产业在云南地区的发展,种植制度不断变革,种植面积不断扩大,苗木调运频繁,加快了病害的传播和蔓延,使得根腐病在各苹果种植区发生危害严重。根腐病在作物地下部分发生,苹果作为多年生乔木发病周期长,发病初期不易识别,发病后期难于药剂防治。此外,根腐病发生后出现严重的连作障碍,成为果园毁灭性病害。In order to clarify the occurrence of root rot disease in Yunnan apple producing areas,the types of pathogens and the existence of new pathogens,this study aims to investigate the presence of root rot pathogens in Yunnan apple orchards and identify any potential new pathogens.Disease samples were collected from apple producing areas in Yunnan.The pathogens were obtained by tissue isolation,and the pathogenicity was verified by Koch's rule.The identification of pathogen was preliminarily determined by combining morphology.Furthermore,molecular biology identification was carried out based on the method of internal transcribed spacer and large subunit multi-locus gene joint construction.Phytopythium was isolated from Jian chuan County,Dali City,and Ning lang County,Lijiang City.It was confirmed through pathogenicity determination so that it could cause apple root rot.Through morphology combined with molecular biology characters.It was identified as Phytopythium litorale and P.cucurbitacearum.This study represents the first report of apple root rot caused by the pathogens P.litorale and P.cucurbitacearum in China.The results provide a theoretical basis for the development of monitoring disease occurrence and comprehensive control technology.

关 键 词：防治 Phytopythium 连作障碍 苹果 传播 种植制度 病害 

分 类 号：S432.44[农业科学—植物病理学]