云南省雪茄烟枯萎病病原菌分离与鉴定  

Isolation and identification of the causing agent of cigar wilt in Yunnan province

作　　者：韦吉霞 张定里 郑元仙 王继明 陈小龙 余磊 何元胜 高鹏华 WEI Jixia;ZHANG Dingli;ZHENG Yuanxian;WANG Jiming;CHEN Xiaolong;YU Lei;HE Yuansheng;GAO Penghua(Agricultural College of Kunming University,Yunnan Urban Characteristic Agricultural Engineering Technology Research Center,Kunming 650214,China;Lincang City of Yunnan Tobacco Company,Lincang 677000,China;China Tobacco Henan Industry Company with Limited Liability,Zhengzhou 450000,China)

机构地区：[1]昆明学院农学与生命科学学院,云南都市特色农业工程技术研究中心,昆明650214 [2]云南省烟草公司临沧市公司,临沧677000 [3]河南中烟工业有限责任公司,郑州450000

出　　处：《植物病理学报》2025年第2期356-359,共4页Acta Phytopathologica Sinica

基　　金：中国烟草总公司云南省公司科技项目(2022530900242001)。

摘　　要：雪茄烟(cigar)是烟草(NicotianatabacumL.)的一种类型,具有香气重、劲道足、焦油和烟碱含量少等特点^([1-2])。雪茄烟是我国云南省临沧市主要经济作物,对于改善当地人民经济状况具有举足轻重的作用。随着雪茄烟推广种植面积的不断扩大,连年种植单一品种等原因,根茎类病害对雪茄烟的种植及产业发展所造成的影响日渐严重。Cigar is an important economic crop in Yunnan province.Recently,the wilt disease occurred seriously on cigar in Lincang city,Yunnan province,China.To explore the main pathogenic fungi of cigar wilt,pure strains were obtained from the disease samples by using the tissue isolation method.Twenty-one representative isolates were subsequently obtained and exhibited identical morphological characteristics.All isolates produced large conidia with sickle shap,small conidia with kidney shape,chlamydospores with round or oval shape;A representative isolate(YX39-1)was selected for molecular identification and the rDNA-ITS(GenBank accession No.ON918599),EF-1α(OR808022),β-tubulin(OP053690)and RPB2(OP369287)gene sequences were 99.25%,99.86%,98.42%and 98.17%similar to those of Fusarium solani isolates(MT896771,ON843639,ON366357 and XM_046273849,respectively).Thus,the fungus represented by isolate YX39-1 was identified as F.solani based on cultural and morphological features.Pathogenic test showed that all inoculated plants exhibited symptoms similar to those observed in the field and YX39-1 was a strong pathogenic strain.The morphological and molecular identification of the fungal reisolated from inoculated plants confirmed its identity as F.solani,fulfilling Koch’s postulates.This is the first report of cigar wilt disease caused by F.solani causing in Lincang,Yunnan Province,China,which provides important information on the pathogen for disease management.

关 键 词：雪茄烟 枯萎病 鉴定 病原菌 烟草 分离 临沧市 

分 类 号：S435.79[农业科学—农业昆虫与害虫防治]