玉米黄花叶病毒CP基因的原核表达及抗血清制备  

Prokaryotic expression and antiserum preparation of the coat protein of maize yellow mosaic virus

作　　者：史亚娟[1] 谢莉娜 孙书豪 崔荧钧 李好海 周涛 陈琳琳[1] 施艳[1] 杨雪 李洪连[1,3,4] SHI Yajuan;XIE Lina;SUN Shuhao;CUI Yingjun;LI Haohai;ZHOU Tao;CHEN Linlin;SHI Yan;YANG Xue;LI Honglian(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;Crop Science Postdoctoral Programme of Henan Agricultural University,Zhengzhou 450002,China;Henan Food Crops Collaborative Innovation Center,Zhengzhou 450002,China;Key Laboratory of Wheat and Maize Crop Science,Zhengzhou 450002,China;Henan Association for the Promotion of New Plant Protection Techniques;Henan Plant Protection and Quarantine Atation,Zhengzhou 450002,China;College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学作物学博士后流动站,郑州450002 [3]河南省粮食作物协同创新中心,郑州450002 [4]小麦玉米作物学国家重点实验室,郑州450002 [5]河南省植物保护新技术推广协会 [6]河南省植物保护检疫站,郑州450002 [7]中国农业大学植物保护学院,北京100193

出　　处：《植物病理学报》2025年第2期360-364,共5页Acta Phytopathologica Sinica

基　　金：河南省重点研发专项(231111111100);河南省玉米产业技术体系植保岗位科学家科研专项(HARS-22-02-C3);河南农业大学科技创新项目(KJCX2021A13)。

摘　　要：0引言玉米黄花叶病毒(maize yellow mosaic virus,MaYMV)是马铃薯卷叶病毒属(Polerovirus)的新成员^([1])。MaYMV基因组全长5642nt,为正义单链RNA,包含7个开放阅读框(ORF),其中ORF3编码一个23.6kDa的蛋白,为MaYMV外壳蛋白(coat protein,CP)。2016年,我国首次报道了MaYMV病毒为害玉米造成黄花叶症状^([2]),随后在国外多地也报道了该病毒的危害.Maize yellow mosaic virus(MaYMV)as a newly identified Polerovirus causes leaf reddening in maize and yellow dwarf in wheat,leading to severe economic loss.In this study,the CP gene was amplified by RT-PCR from MaYMV-infected maize leaves,cloned into a prokaryotic expression vector pET28a(+)and transformed into the Escherichia coli strain BL21.The recombinant CP protein was expressed after induction with IPTG and used to prepare the antiserum by injection into New Zealand white rabbits.In-ELISA results showed that the titer of the antiserum was 1.024×10^(6).Dot-ELISA and Western blot analysis confirmed that the sensitivity of the antiserum to the MaYMV leaf sap was 1:500,and the antiserum reacted specifically with MaYMV CP.These results indicate that the antiserum of MaYMV CP could be used for rapid detection of MaYMV.This study will provide an important basis for the monitoring and prediction of MaYMV.

关 键 词：外壳蛋白 CP基因 玉米黄花叶病毒 MaYMV 

分 类 号：S432.44[农业科学—植物病理学]