基于JNK/P38 MAPK通路探讨白藜芦醇对酒精依赖大鼠肝脏的保护作用  

The protective effect of resveratrol on the liver of alcohol-dependent rats based on JNK/P38 MAPK pathway

作　　者：衣浩然 韩智学 刘琦 郑天翼 薛继婷 刘丽 岳辉 杨勇 李齐 刘洪凤 YI Haoran;HAN Zhixue;LIU Qi;ZHENG Tianyi;XUE Jiting;LIU Li;YUE Hui;YANG Yong;LI Qi;LIU Hongfeng(Mudanjiang Medical University,Mudanjiang 157011,China;Mudanjiang Medical University Affiliated Hongqi Hospital)

机构地区：[1]牡丹江医科大学,黑龙江牡丹江157011 [2]牡丹江医科大学附属红旗医院

出　　处：《包头医学院学报》2025年第4期29-33,共5页Journal of Baotou Medical College

基　　金：黑龙江省自然科学基金项目(LH2020H075);黑龙江省教育厅高校基本科研业务经费项目(2022-KYYWF-0706);黑龙江省卫生健康委科技计划项目(20220202020597);牡丹江医学院导师科研专项计划项目(YJSZX2022035);牡丹江医学院导师科研专项计划项目(YJSZX2022030)。

摘　　要：目的:探讨白藜芦醇(resveratrol,RES)对酒精依赖大鼠致肝脏损伤的保护作用。方法:根据随机数字表法将SD大鼠设立饮酒组和空白对照组,饮酒组大鼠给予隔日间断双瓶自由饮20%酒精建立酒精依赖模型,空白对照组大鼠双瓶自由饮水。建模成功后将饮酒组大鼠随机分为酒精依赖模型组(alcohol dependence,AD),RES低、中、高剂量给药组(Low,Middle,High)。治疗结束后取肝组织分别检测各组大鼠过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(super oxide dismutase,SOD)活性,采用Western Blot检测p38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)、C-Jun氨基末端激酶(cJun N-terminal kinase,JNK)及p-P38 MAPK、p-JNK的蛋白表达。结果:与空白对照组相比,AD组大鼠肝组织MDA活性升高(P<0.05),CAT、GSH-PX、SOD活性均降低(P<0.05),p-P38、p-JNK的蛋白表达明显升高(P<0.05);与AD组相比,给药后RES中、高剂量组MDA活性明显降低(P<0.05),CAT、GSH-PX、SOD活性明显升高(P<0.05),p-P38、p-JNK蛋白表达明显降低(P<0.05)结论:RES对酒精依赖所致的大鼠肝组织损伤具有改善作用,并可能通过抑制JNK/P38信号通路中JNK、P38蛋白的磷酸化,降低肝组织氧化应激损伤,对酒精依赖大鼠肝脏起到保护作用。Objective:To investigate the protective effect of resveratrol(RES)on liver injury induced by alcohol dependence in rats.Methods:According to the random number table method,SD rats were divided into drinking group and blank control group.The rats in the drinking group were given two bottles of free drinking 20%alcohol every other day to establish an alcohol dependence model,and the rats in the blank control group were given two bottles of free drinking water.After successful modeling,the rats in the drinking group were randomly divided into alcohol dependence model group(AD),RES low,medium and high dose administration groups(Low,Middle,High).After treatment,the activities of catalase(CAT),glutathione peroxidase(GSH-PX),malondialdehyde(MDA)and super oxide dismutase(SOD)in liver tissues were detected.Western Blot was used to detect the protein expression of p38 mitogen-activated protein kinase(MAPK),c-Jun N-terminal kinase(JNK),p-P38 MAPK and p-JNK.Results:Compared with the blank control group,the activity of MDA in liver tissue of AD group was increased(P<0.05),the activities of CAT,GSH-PX and SOD were decreased(P<0.05),and the protein expression of p-P38 and p-JNK was significantly increased(P<0.05).Compared with the AD group,the activity of MDA in the middle and high dose RES groups was significantly decreased(P<0.05),the activities of CAT,GSH-PX and SOD were significantly increased(P<0.05),and the expression of p-P38 and p-JNK protein was significantly decreased(P<0.05).Conclusion:RES can improve the liver injury induced by alcohol dependence in rats,and may reduce the oxidative stress injury of liver tissue by inhibiting the phosphorylation of JNK and P38 proteins in JNK/P38 signaling pathway,and play a protective role in the liver of alcohol dependent rats.

关 键 词：酒精依赖 白藜芦醇 JNK/P38 MAPK信号通路 

分 类 号：R285.5[医药卫生—中药学]