基于均匀插值的浮游藻类规范光谱库构建方法及应用  

Construction Method and Application of a Standardized Phytoplankton Spectral Library Based on Uniform Interpolation

作　　者：张小玲[1] 王思琦 赵南京[2] 殷高方[2] 董鸣 王翔 张胜俊 陈玮杰 ZHANG Xiao-ling;WANG Si-qi;ZHAO Nan-jing;YIN Gao-fang;DONG Ming;WANG Xiang;ZHANG Sheng-jun;CHEN Wei-jie(Anhui University,Hefei 230039,China;Key Laboratory of Environmental Optics and Technology,Anhui Institute of Optic and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Institute of Environment Hefei Comprehensive National Science Center,Hefei 230022,China;Anhui Polytechnic University,Wuhu 241000,China)

机构地区：[1]安徽大学,安徽合肥230039 [2]中国科学院环境光学与技术重点实验室,中国科学院安徽光学精密机械研究所,安徽合肥230031 [3]合肥综合性国家科学中心环境研究院,安徽合肥230022 [4]安徽工程大学,安徽芜湖241000

出　　处：《光谱学与光谱分析》2025年第5期1225-1235,共11页Spectroscopy and Spectral Analysis

基　　金：国家重点研发计划项目(2022YFC3103901,2021YFC3200102);国家自然科学基金项目(62005001,62375270,42206198);安徽省生态环境科研项目(2023hb0012,2023hb0011);安徽省科技创新平台重大科技项目(S202305a12020004);中国仪器仪表学会科学仪器托举计划项目(CISTJ2024)资助。

摘　　要：浮游藻类是海洋生态系统中最重要的生产者。离散三维荧光光谱法解决了连续三维荧光光谱仪运行条件要求高的问题,能够满足浮游藻类的在线与原位监测需求。但活体荧光不稳定性以及色素组成相似性导致的不同藻类光谱之间离散三维荧光光谱差异降低的问题,限制了浮游藻离散三维荧光光谱法的实际应用。本工作研究了不同生境条件下硅藻和甲藻三维荧光光谱特征,分析了常见的浓度归一化平均值(以下称平均法)规范光谱库构建方式的局限性,在此基础上提出了一种基于均匀插值的规范光谱库构建方法。通过实验分别研究了浮游藻类规范光谱库的最佳插值范围和最优插值数目,结果表明,M±2S范围的平均覆盖率达88%,平均相关系数达0.98,是光谱库的最优插值的范围。当插值数目为10条时,测量准确性比平均法提高了近13%,运算时间为0.4948 s,是兼顾准确性和运算效率的最优插值数目。基于此,构建基于均匀插值的硅藻和甲藻类规范光谱库,结合最小二乘多元线性回归分析,对实验室配置的浓度已知藻类样品进行三维荧光光谱解析,并与平均值法解析结果进行比较。结果表明,采用均匀插值法构建的规范光谱库,对5种不同浓度梯度藻样本的测量值与标准叶绿素a浓度之间的相关系数(k值)范围为0.828~1.149,决定系数(R2)在0.616~0.953之间。对硅藻门识别失败的样本数量从平均值法的21个显著减少至仅2个,对硅藻和甲藻的解析相对误差平均值分别为36.9%和30.7%,较平均值法分别降低了20.6%和19.0%,表明基于均匀插值的规范光谱库构建方法非常有效地提高了硅藻和甲藻的定量解析精度。将此建库方式分别扩展至蓝藻、绿藻和隐藻,并应用于海水叶绿素原位监测仪(AFA)的南海试验。本研究为浮游藻类快速准确测量提供了一种有效的技术方法,也为未来海洋生态监测和环境保护�Phytoplankton are crucial producers in marine ecosystems.The discrete three-dimensional fluorescence spectroscopy method addresses the high operational requirements of continuous three-dimensional fluorescence spectrophotometers,thus meeting the needs for online and in-situ phytoplankton monitoring.However,challenges persist due to the instability of living fluorescence and the similarity in pigment composition among different algal species.This reduces the spectral differentiation between algal groups in discrete three-dimensional fluorescence spectroscopy and limits its practical application.This study investigates the three-dimensional fluorescence spectral characteristics of Bacillariophyta and Dinophyta under various environmental conditions.It analyzes the limitations of the commonly used average concentration-normalized standardized spectral library construction methods(hereinafter referred to as the“average method”).Based on this,a standardized phytoplankton spectral library construction method is proposed using uniform interpolation(hereinafter referred to as the“interpolation method”).The optimal interpolation range and number of interpolations for the phytoplankton standardized spectral library were determined through experiments.The results show that an average coverage rate of 88%and an average correlation coefficient of 0.98 are achieved within the range of M±2S,which is identified as the optimal interpolation range.With 10 interpolations,measurement accuracy improves by nearly 13%compared to the average method,and the computation time is only 0.4948 seconds,marking it as the optimal number of interpolations considering both accuracy and computational efficiency.Accordingly,a standardized spectral library for Bacillariophyta and Dinophyta was constructed based on uniform interpolation.Combined with non-negative least squares linear regression analysis,this method was used to interpret the three-dimensional fluorescence spectra of a series of algal samples with known concentrations,and the i

关 键 词：浮游藻类 荧光法 光谱库构建 均匀插值 生态安全 

分 类 号：O433.4[机械工程—光学工程]