基于网络药理学探讨黄芪-丹参干预肾纤维化的作用机制  

Network Pharmacology-based Discussion on the Mechanism of Intervention of Renal Fibrosis with Huangqi-Danshen Couplet Medicines

作　　者：石晓冬 卢登勇 吴慧敏 陈宇珊 左金巾 钟建[2] SHI Xiaodong;LU Dengyong;WU Huimin;CHEN Yushan;ZUO Jinjin;ZHONG Jian(Graduate School of Guangxi University of Chinese Medicine,Nanning 530000,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学研究生院,广西南宁530000 [2]广西中医药大学第一附属医院,广西南宁530001

出　　处：《西部中医药》2025年第4期53-58,共6页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金地区基金项目(81760807)。

摘　　要：目的:探究黄芪-丹参药对防治肾纤维化的作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索黄芪、丹参两味中药的活性成分和作用靶点;利用人类基因数据库(the human gene database,Gene-Cards)及在线人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)检索与肾纤维化相关的基因,并使用UniProt数据库校正靶点对应的基因名称;通过Cytoscape 3.6.0软件构建黄芪-丹参药对的活性成分-靶点网络及蛋白-蛋白互作(protein-protein interactions,PPI)网络,筛选出核心活性成分及靶点;使用核心靶基因导入基因功能注释数据库(the database for annotation visualization and integrated discovery,DAVID)进行基因本体论(gene ontology,GO)功能富集分析和京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果:得到黄芪-丹参干预肾纤维化的有效活性成分85个,有效作用靶点1297个;富集分析得出作用机制可能与PI3K/AKT、HIF-1、细胞凋亡、甲状腺激素、MAPK、钙、NF-κB、血管内皮生长因子等信号通路有关,且IL-6、CASP3、MAPK8、VEGFA、EGFR、MYC、CCND1、ESR1、FOS、ERBB2、AR、RELA、PPARG等可能是芪-丹参干预肾纤维化的重要靶点基因。结论:黄芪-丹参药对干预肾纤维化具有多成分-多靶点-多通路的特点。Objective:To survey the mechanism of Huangqi(Astragali radix)-Danshen(Salviae miltiorrhizae radix et rhizoma)in the prevention and treatment of renal fibrosis.Methods:Active ingredients and the targets of action of Huangqi and Danshen were searched from TCMSP;GeneCards and OMIM were utilized to search renal fibrosis-related genes,and UniProt database was used to correct the names of the genes corresponding to the targets;Cytoscape 3.6.0 software was applied to construct active ingredients-target network and PPI interaction of the couplet medicines,for the screening of the core active ingredients and targets;DAVID was used to perform GO and KEGG enrichment analysis.Results:All 85 effective active ingredients and 1297 effective targets of action were obtained from the couplet medicines;the enrichment analysis revealed that the mechanism might be associated to the signaling pathways including PI3K/AKT,HIF-1,cellular apoptosis,thyroid hormone,MAPK,calcium,NF-κB and vascular endothelial growth factors,and IL-6,CASP3,MAPK8,VEGFA,EGFR,MYC,CCND1,ESR1,FOS,ERBB2,AR,RELA and PPARG might be the important target genes for the intervention of renal fibrosis with the couplet medicines.Conclusion:Huangqi-Danshen couplet medicines have the characteristics of multi-component,multi-target and multi-pathway for the intervention of renal fibrosis.

关 键 词：肾纤维化 网络药理学 黄芪 丹参 

分 类 号：R285[医药卫生—中药学]