基于代谢组学探究形神共养对糖尿病合并抑郁症大鼠的影响  

Exploring the effects of preservation from both physique and spirit on rats with diabetes mellitus combined with de-pression based on metabolomics

作　　者：张先庚 田静 张汗玉 杨丽萍[3] 梁小利 王红艳 ZHANG Xiangeng;TIAN Jing;ZHANG Hanyu(Sichuan Nursing Vocational College,Chengdu 610000,China;West China Second University Hospital,Sichuan University,Chengdu 610000,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610000,China)

机构地区：[1]四川护理职业学院,四川成都610000 [2]四川大学华西第二医院,四川成都610000 [3]成都中医药大学,四川成都610000

出　　处：《四川中医》2025年第4期134-142,共9页Journal of Sichuan of Traditional Chinese Medicine

基　　金：四川省科技厅四川省自然科学基金项目(编号:2023NSFSC1791);四川省中医药管理局中医药科研专项课题(编号:2023MS238);国家中医药管理局中医药养生健康产业发展重点研究室开放基金资助(编号:GZ2022013);四川养老与老年健康协同创新中心联合基金项目(编号:YLKYZD2204)。

摘　　要：目的:本研究拟从血液代谢的角度探究形神共养对糖尿病合并抑郁症雄性健康(SD)大鼠代谢产物的影响。方法:45只雄性SD大鼠随机分为空白组、模型组、中药组、形神共养组。除空白组外,其余各组通过注射链脲佐菌素(STZ)和采用21天慢性不可预知性应激(CUMS)方式进行糖尿病合并抑郁症造模,造模成功后,进行每日的干预。空白组和模型组给予5mL/kg的生理盐水;中药组给予一贯煎6.38g/kg;形神共养组给予丰富环境和一贯煎6.38g/kg。干预6周结束后,取血液样本。结果:非靶代谢组学结果表明,与空白组相比,模型组的血清代谢物中神经节苷脂GA2(d18:1/18:0)的水平表达上调。与模型组相比,形神共养组的血清代谢物中神经节苷脂GA2(d18:1/18:0)丰度水平下调。与空白组相比,模型组显著上调了鞘脂类代谢产物的丰度。与模型组相比,形神共养组显著下调了鞘脂类代谢产物的丰度水平。此外,形神共养组还可以调节各种磷脂类和氨基酸类代谢产物的丰度。四组间差异显著的KEGG途径,主要参与新陈代谢过程。有5条代谢途径影响较大,包括半乳糖代谢、ABC转运体、癌症中的中枢碳代谢、氨基酰-tRNA的生物合成、戊糖和葡萄糖醛酸的相互转化、逆行内源性大麻素信号传导。结论:中药组与形神共养组均能在不同程度显著调节糖尿病合并抑郁症大鼠的代谢紊乱。形神共养组可改变糖尿病合并抑郁症大鼠的代谢谱,调节新陈代谢等通路活性,促进磷脂类、鞘脂类、氨基酸类等代谢产物的丰度显著增加,或将对糖尿病合并抑郁症大鼠血糖水平以及抑郁症状改变产生有益影响。Objective:This study aims to explore the effects of preservation from both physique and spirit on the metabolic products of diabetic rats with depression from the perspective of blood metabolism.Methods:Forty-five male SD rats were randomly divided into the blank group,the model group,the traditional Chinese medicine group,and the preservation from both physique and spirit group.Except for the blank group,the other groups were modeled by injecting streptozotocin(STZ)and using 21-day chronic unpredictable mild stress(CUMS).After successful modeling,daily intervention was carried out.The blank group and the model group were given 5ml/kg of normal saline;the traditional Chinese medicine group was given the decoction of consistent 6.38g/kg;the preservation from both physique and spirit group was given a rich environment and the decoction of consistent 6.38g/kg.After 6weeks of intervention,blood samples were taken.Results:The non-target metabolomics results showed that compared with the blank group,the level of expression of glycosphingolipid GA2(d18:1/18:0)in the serum metabolites of the model group was up-regulated.Compared with the model group,the abundance level of glycosphingolipid GA2(d18:1/18:0)in the serum metabolites of the preservation from both physique and spirit group was down-regulated.Compared with the blank group,the abundance level of sphingolipid metabolites in the model group was significantly up-regulated.Compared with the model group,the abundance level of sphingolipid metabolites in the preservation from both physique and spirit group was significantly down-regulated.In addition,the preservation from both physique and spirit group could also regulate the abundance levels of various phospholipid and amino acid metabolites.The significant differences among the four groups were mainly in the KEGG pathways involved in metabolic processes.Five metabolic pathways had a significant impact,including galactose metabo-lism,ABC transporters,central carbon metabolism in cancer,biosynthesis of aminoacyl-tRNA,

关 键 词：代谢组学 形神共养 糖尿病合并抑郁症 代谢产物 

分 类 号：R285[医药卫生—中药学]