辛前甘桔汤对白介素13诱导的鼻黏膜上皮细胞miR-146a/TLR4信号通路及黏蛋白分泌的影响  

Effect of Xinqian Ganjie decoction on miR-146a/TLR4signaling pathway and mucin secretion of nasal mucosal epi-thelial cells induced by IL-13

作　　者：施陈燕 孙茹 滕磊[1] 蔡蔚然 袁波[1] 董雪林 鞠晗 丁旭 暴喜明 张治军[1] SHI Chenyan;SUN Ru;TENG Lei(Department of Otolaryngology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院耳鼻咽喉科,上海201203

出　　处：《四川中医》2025年第4期149-153,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金委员会面上项目、青年项目(编号:82174447,82104940)。

摘　　要：目的:研究辛前甘桔汤对白介素13(Interleulin-13,IL-13)诱导的鼻黏膜上皮细胞微小RNA-146a(miR-146a)/Toll样受体4(Toll-like receptor4,TLR4)信号通路及黏蛋白分泌的影响。方法:将18只SD大鼠随机分成对照组、模型组和中药组,每组6只,制备含药血清;培养鼻黏膜上皮细胞,将细胞分为对照组(对照组血清处理)、模型组(10ng/mL IL-13处理24h+对照组血清)、中药组(10ng/mL IL-13处理24h+辛前甘桔汤血清),观察鼻黏膜上皮细胞形态,采用酶联免疫吸附试验(ELISA)法检测白介素1β(Interleulin-1β,IL-1β)、白介素6(Interleulin-6,IL-6)、肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)、黏蛋白5AC(Mucin 5AC,MUC5AC)表达,qRT-PCR法检测miR-146a、TLR4、髓样分化因子(Myeloiddifferentiationfactor88,MyD88)、MUC5AC的mRNA表达。结果:与对照组相比,模型组IL-1β、IL-6、TNF-α、MUC5AC表达明显升高(P<0.01),miR-146a mRNA表达减少(P<0.05),TLR4、MyD88、MUC5AC mRNA表达明显升高(P<0.01);与模型组比较,中药组IL-1β、IL-6、TNF-α、MUC5AC表达明显降低(P<0.01),miR-146a mRNA表达增加(P<0.01),TLR4、MyD88、MUC5AC mRNA表达明显降低(P<0.01)。结论:辛前甘桔汤通过调节miR-146a调控TLR4信号通路,从而减少炎性细胞因子IL-1β、IL-6、TNF-α及黏蛋白MUC5AC,发挥抗炎、减少黏液分泌作用。Objective:To study the effect of Xinqian Ganjie Decoction on mir-146a/TLR4signaling pathway and mucin se-cretion of nasal mucosal epithelial cells induced by IL-13.Methods:Eighteen SD rats were randomly divided into control group,model group and traditional Chinese medicine group,with 6rats in each group.Nasal epithelial cells were cultured and divided in-to control group(treated with 10ng/mL IL-13for 24h),model group(control group serum after being treated with 10ng/mL IL-13for 24h),traditional Chinese medicine group(Xinqian Ganjie Decoction serum after being treated with 10ng/mL IL-13 for 24h).The morphology of nasal epithelial cells was observed.The expression of IL-1β,IL-6,TNF-α,MUC5AC was detected by ELISA.The expression of miR-146a,TLR4,MyD88,MUC5AC mRNA was detected by qRT-PCR.Results:Compared with the control group,the expression of IL-1β,IL-6,TNF-αand MUC5AC in the model group was significantly increased(P<0.01),the expression of miR-146a mRNA was decreased(P<0.05),and the expression of TLR4,MyD88and MUC5AC mRNA was significantly increased(P<0.01).Compared with the model group,the expression of IL-1β,IL-6,TNF-αand MUC5AC in the traditional Chinese medicine group was significantly decreased(P<0.01),the expression of miR-146a mRNA was increased(P<0.01),and the expression of TLR4,MyD88and MUC5AC mRNA was significantly decreased(P<0.01).Conclusion:Xinqian Ganjie Decoction regulates TLR4signaling pathway by regulating miR-146a,thereby reducing inflammatory cytokines IL-1β,IL-6,TNF-αand mucin MUC5AC,playing an anti-inflammatory and reducing mucus secretion effect.

关 键 词：辛前甘桔汤 鼻黏膜上皮细胞 miR-146a/TLR4信号通路 黏蛋白分泌 

分 类 号：R285[医药卫生—中药学]