基于网络药理学探究健脾止动汤治疗儿童抽动障碍的作用机制  

Mechanism of Jianpi Zhidong Decoction in the treatment of TICdisorder based on network pharmacology

作　　者：祖夏琳 吕广引 刘晓芳[1] ZU Xia-lin;LV Guangyin;LIU Xiaofang(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学东方医院,北京100078

出　　处：《四川中医》2025年第4期159-165,共7页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学青年科学基金资助项目(编号:82004422);儿科专委会青年医师创新发展项目(编号:2018-JYBZZ-JS127)。

摘　　要：目的:本研究运用网络药理学的方法,深入剖析健脾止动汤在治疗儿童抽动障碍领域的效用原理,力求为其临床应用奠定理论基础。方法:在TCMSP数据库中检索并筛选出健脾止动汤的药物活性成分,筛选标准设定为口服生物利用度(OB)不小于30%,且类药性(DL)不小于0.18,借助SwissTargetPrediction数据库,对这些活性成分进行靶点基因的预测分析;在Gene Cards、OMIM、TTD数据库中,以“TIC Disorder”作为检索关键词,收集TD疾病的靶点信息;通过绘制韦恩图,确定药物成分与疾病之间的交集靶点;构建PPI网络,并对核心靶点进行深入分析;进行GO功能和KEGG通路富集分析;构建“药物-成分-靶点-疾病”图。结果:健脾止动汤在治疗儿童抽动障碍方面的主要活性成分包括异去氢钩藤碱、毛钩藤碱、育亨宾等,核心靶点基因则涵盖AKT1、GAPDH、IL-6、TNF、TP53等。在KEGG通路方面,健脾止动汤主要通过调控神经活性配体与受体的相互作用、钙信号通路,5-羟色胺能突触以及肿瘤信号通路,环磷酸腺苷(cAMP)信号通路及丝裂原活化蛋白激酶(MAPK)信号通路等,实现对儿童抽动障碍的有效治疗。结论:健脾止动汤通过多成分作用于多个靶点进而调控多条通路,实现对儿童抽动障碍的有效治疗。Objective:This study aims to explore the therapeutic mechanisms of Jianpi Zhidong Decoction in the treatment of TIC Disorder(TD)using the methods of network pharmacology,thereby providing theoretical support for clinical practice.Meth-ods:Active ingredients of Jianpi Zhidong Decoction were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),with the criteria set as oral bioavailability(OB)not less than 30%and drug-likeness(DL)not less than 0.18.The target genes of these active components were predicted using the SwissTargetPrediction database.The target information of TD was collected from the GeneCards,OMIM,and Therapeutic Target Database(TTD),using“TIC Disorder”as the search keyword.The intersection targets between the drug components and the disease were identified by con-structing a Venn diagram.A PPI network was constructed,and the core targets were analyzed in depth.GO function and KEGG pathway enrichment analyses were performed.Finally,a“drug-component-target-disease”network was constructed.Results:The main active components of Jianpi Zhidong Decoction in the treatment of TD include coryincine,hirsutine,yohimbine,among others.The core target genes include AKT1,GAPDH,IL-6,TNF,and TP53.In terms of KEGG pathways,Jianpi Zhidong De-coction mainly regulates the Neuroactive ligand-receptor interaction,Calcium signaling pathway,Serotonergic synapse,Pathways in cancer,cAMP signaling pathway,and MAPK signaling pathway to effectively treat TD.Conclusion:Jianpi Zhidong Decoction exerts its therapeutic effect on TD through the action of multiple components on multiple targets,thereby regulating multiple signa-ling pathways.

关 键 词：网络药理学 健脾止动汤 抽动障碍 作用机制 

分 类 号：R285[医药卫生—中药学]