桔梗白花和紫花发育过程基因差异表达研究  

Study on Differential Gene Expression during the Development of White and Purple Flowers of Platycodon grandiflorus

作　　者：齐书娅 严一字[1] 金卓 吴松权[1] Qi Shuya;Yan Yizi;Jin Zhuo;Wu Songquan(Agricultural College,Yanbian University,Yanji 133000,Jilin,China)

机构地区：[1]延边大学农学院,吉林延吉133000

出　　处：《作物杂志》2025年第2期54-65,共12页Crops

基　　金：吉林省科技厅重点研发项目“高品质道地药材桔梗的生态种植技术集成”(20220204048YY)。

摘　　要：为挖掘影响桔梗紫花花色形成基因和途径,利用百迈客云平台BMKCloud对桔梗白花和紫花3个时期(花蕾期、转色期、开放期)进行了转录组测序,筛选桔梗花青素代谢相关差异表达基因。筛选到5个KEGG通路与花青素代谢相关。本试验共筛选出11个参与花青素生物合成的候选基因,包括9个关键酶基因(HCT、CHI、DFR、F3′5′H、ANS、3'GT、CCR、NAR、HPPR)和2个糖基酶基因(β-葡萄糖苷酶BoGH3B和UGT79B6),持续表达影响桔梗紫花花色形成。本研究构建了桔梗紫花形成的表达调控通路,筛选出调控桔梗紫花形成的候选基因,为研究桔梗紫花的遗传和分子机制研究提供参考。To explore the genes and pathways influencing the color formation of Platycodon grandiflorus purple flowers,the BMKCloud platform from Biomarker Technologies was used to conduct transcriptome sequencing on both white and purple P.grandiflorus flowers at three stages(bud,color changing,and blooming).The differentially expressed genes related to anthocyanin metabolism in P.grandiflorus were screened.Five KEGG pathways related to anthocyanin metabolism were screened out.A total of 11 candidate genes involved in anthocyanin biosynthesis were screened out,including nine key enzyme genes(HCT,CHI,DFR,F3'5'H,ANS,3'GT,CCR,NAR,HPPR)and two glycosylase genes(β-glucosidase BoGH3B and UGT79B6),and their continuous expression affects the formation of purple flowers of P.grandiflorus.This research constructed an expression regulation pathway for the formation of P.grandiflorus purple flowers and identified the candidate genes involved in this process,thus providing new insights for the genetic and molecular mechanism research of P.grandiflorus purple flowers.

关 键 词：转录组 花青素 桔梗 花色 差异表达基因 

分 类 号：S567.1[农业科学—中草药栽培]