文蛤产后能量代谢变化分析  

Analysis of the energy metabolism-related changes in the clam Meretrix petechialis after spawning

作　　者：张双丽 王鸿霞[1] 刘保忠[1,2,3] ZHANG Shuangli;WANG Hongxia;LIU Baozhong(CAS Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Science,Qingdao 266071,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shandong Dehe Mingxing Biotechnology Co.,Ltd.,Weifang 261309,China)

机构地区：[1]中国科学院海洋研究所实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院大学,北京100049 [3]山东得和明兴生物科技有限公司,山东潍坊261309

出　　处：《海洋科学》2025年第1期14-20,共7页Marine Sciences

基　　金：现代农业产业技术体系(CARS-49)和山东省泰山产业领军专项资助。

摘　　要：动物生殖是一项需要消耗大量能量的生命活动。为探究雌、雄文蛤配子排放后是否存在能量代谢差异,我们对产卵排精后的雌、雄文蛤在连续时间内进行了生理指标测定,并对足组织中糖原和葡萄糖含量及糖代谢途径中关键的限速酶活性进行了检测。结果显示,排卵后雌蛤的耗氧率(ROR)、排氨率(RNR)、摄食率(RIR)与排粪率(RFER)均高于排精后的雄蛤,表明雌蛤的生殖生理活动强度高于雄蛤,因而繁殖代价更大,产后需要摄入更多的能量用以恢复其生理状态;雌、雄文蛤的氧氮比在产后一直高于24,提示代谢底物仍以碳水化合物和脂肪为主。文蛤的葡萄糖含量在产后迅速上升然后逐渐恢复,配子排放前后雌、雄文蛤的己糖激酶(HK)活性变化不大,雄蛤的磷酸果糖激酶(PFK)活性在生产前后保持稳定,雌蛤PFK活性在排卵后显著下降,表明雌蛤的糖代谢活动受到了一定程度的抑制。研究结果有助于理解生殖活动对不同性别文蛤能量代谢影响的差异,为文蛤健康养殖提供科学参考。The reproductive process in animals is a life activity that requires significant amounts of energy.To investigate the potential differences in energy metabolism between the male and female clams Meretrix petechialis after spawning,we monitored their physiological indicators at regular intervals and analyzed the glycogen and glucose levels in the foot tissues,as well as the activities of key rate-limiting enzymes involved in sugar metabolism.Our findings revealed that the oxygen consumption,ammonia excretion,feeding,and fecal excretion rates after spawning were higher in female clams compared to males,indicating an enhanced reproductive cost and increased energy intake to restore the physiological state.Both females and males consistently exhibited oxygen–nitrogen ratios>24 after spawning,signifying carbohydrates and fats as the primary metabolic substrates.Glucose levels elevated rapidly after spawning and then gradually returned to normal,indicating dynamic changes and recovery processes of sugar compounds after spawning.The activity of hexokinase in males and females remained relatively stable before and after spawning.However,that of phosphofructokinase in females declined markedly after spawning,suggesting a specific suppression of carbohydrate metabolism.These findings provide insights into the differential impacts of reproduction on energy metabolism between genders and have implications for sustainable clam aquaculture.

关 键 词：文蛤 生殖 性别差异 生理指标 糖代谢 

分 类 号：Q344[生物学—遗传学]