AMF和ZNC对黄瓜和番茄幼苗生长以及耐低温弱光能力的影响  

AMF and ZNC on Cucumber and Tomato Seedlings Growth and Low Temperature and Low Light Tolerance

作　　者：马澳纪 付雨萱 许丰雅 张大龙 魏珉[1] 史庆华[1] 李岩[1] MA Ao-ji;FU Yu-xuan;XU Feng-ya;ZHANG Da-long;WEI Min;SHI Qing-hua;LI Yan(College of Horticultural Science and Engineering/Shandong Agricultural University,Tai'an 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《山东农业大学学报(自然科学版)》2025年第2期313-319,共7页Journal of Shandong Agricultural University:Natural Science Edition

基　　金：山东省重点研发计划(2022TZXD0025);山东省蔬菜产业技术体系(SDAIT-05)。

摘　　要：本试验以黄瓜‘新泰密刺’和番茄‘D2856’为试材,研究在低温弱光(15℃/8℃,80μmol·m^(-2)·s^(-1),low temperature and low light,LL)下,丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)和宛氏拟青霉(Paecilomyces variotii)提取物智能聪(ZNC)对黄瓜和番茄幼苗生长及其抗氧化系统活性的影响。幼苗两叶一心时LL处理7 d,LL下根施基质重量比1%AMF(LL+AMF)和喷施10 mL 10 ng·mL^(-1) ZNC(LL+ZNC),以正常温光为对照(28℃/18℃,600μmol·m^(-2)·s^(-1),CK)。结果表明,与CK相比,LL显著降低黄瓜和番茄壮苗指数和根系活力,抑制植株生长,且黄瓜和番茄幼苗SOD、POD活性及可溶性糖和蛋白含量增加,说明LL引起植株膜脂过氧化。LL下施用AMF和ZNC显著提高黄瓜和番茄的可溶性糖含量以及POD和CAT活性。其中,LL下施用AMF和ZNC黄瓜的壮苗指数显著提高,较LL分别提高19.4%和11.1%;LL+AMF和LL+ZNC番茄幼苗根系活力及可溶性糖含量较LL分别提高22.7%、14.8%和31.9%、26.9%。综上所述,本试验条件下AMF和ZNC可通过提高黄瓜和番茄幼苗抗氧化酶活性以及渗透调节能力,减轻低温弱光造成的植株膜脂过氧化水平,进而缓解对黄瓜和番茄幼苗的生长抑制。This study used cucumber'Xintai Mici'and tomato'D2856'as test materials to investigate the effects of Arbuscular mycorrhizal fungi(AMF)and the extract of Paecilomyces variotii,known as Zhineng Cong(ZNC),on the growth and antioxidant system activity of cucumber and tomato seedlings under low temperature and low light conditions(15℃/8℃,80μmol·m^(−2)·s^(−1),LL).Seedlings were treated with LL for 7 days at two leaves stage,and rootapplied substrate weight ratio of 1%AMF(LL+AMF)and sprayed with 10 mL of 10 ng·mL^(−1) ZNC(LL+ZNC)under LL,with normal temperature and light as the control(28℃/18℃,600μmol·m^(−2)·s^(−1),CK).The results showed that LL significantly reduced the seedling index and root activity of cucumber and tomato,inhibited plant growth,and increased the activities of SOD and POD and the content of soluble sugar and protein in cucumber and tomato seedlings,indicating that LL caused membrane lipid peroxidation in plants.The application of AMF and ZNC under LL significantly increased the soluble sugar content as well as POD and CAT activities of cucumber and tomato.Among them,the strong seedling index of cucumber was significantly increased by 19.4%and 11.1%with AMF and ZNC,respectively,compared with that of LL;the root vigor and soluble sugar content of tomato seedlings were increased by 22.7%and 14.8%,31.9%and 26.9%.In summary,under the conditions of this experiment,AMF and ZNC could alleviate the membrane lipid peroxidation caused by low temperature and low light stress in cucumber and tomato seedlings by enhancing their antioxidant enzyme activities and osmotic adjustment capacity,thereby mitigating the growth inhibition induced by low temperature and low light.

关 键 词：黄瓜 番茄 低温弱光 丛枝菌根真菌 智能聪 

分 类 号：S641.3[农业科学—蔬菜学]