柳枝稷(Panicum virgatum)莽草酸脱氢酶基因电子克隆及分析  

In Silico Cloning and Analysis of the Shikimate Dehydrogenase Gene from Panicum virgatum

作　　者：夏煌慧 黄建忠[1] XIA Huanghui;HUANG Jianzhong(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117

出　　处：《福建师范大学学报(自然科学版)》2025年第3期97-102,110,共7页Journal of Fujian Normal University:Natural Science Edition

基　　金：国家重点研发计划项目(2022YFD1802104)。

摘　　要：莽草酸脱氢酶(AroE)是芳香族氨基酸合成途径的关键酶,催化3-脱氢莽草酸转化为莽草酸,研究AroE结构有助于揭示其生物催化机制。以玉米(Zea mays)莽草酸脱氢酶基因为探针,筛选拼接高同源EST序列,获得完整cDNA序列。利用ORF预测得到氨基酸序列,对其进行理化性质、蛋白互作、跨膜结构、磷酸化位点及信号肽分析。柳枝稷(Panicum virgatum)AroE由355个氨基酸编码,是一种亲水性的细胞质蛋白,无跨膜结构域和信号肽。进化树表明柳枝稷AroE与禾草(Panicum hallii)同属一类。Shikimate dehydrogenase(AroE)is a key enzyme in the biosynthetic pathway of aromatic amino acids,catalyzing the conversion of 3-dehydroshikimate to shikimate.Analyzing the AroE structure helps elucidate its biocatalytic mechanism.Using the shikimate dehydrogenase gene from Zea mays as a probe,high-homology expressed sequence tag(EST)sequences were screened and assembled to obtain the complete cDNA sequence.The amino acid sequence was predicted through ORF analysis and further examined for its physicochemical properties,protein-protein interactions,transmembrane structure,phosphorylation sites,and signal peptides.The AroE of Panicum virgatum is encoded by 355 amino acids and is a hydrophilic,cytoplasmic protein without transmembrane domains or signal peptides.The phylogenetic tree indicates that the AroE of Panicum virgatum clusters with that of Panicum hallii.

关 键 词：柳枝稷 莽草酸途径 莽草酸脱氢酶 电子克隆 同源性分析 代谢机制 

分 类 号：S567.31[农业科学—中草药栽培]