大麦PRX基因家族全基因组鉴定及其干旱胁迫下的表达分析  

Genome-wide identification of PRX gene family and analysis of their expressions under drought stress in barley

作　　者：陆雯佳 汪军成[1,2] 姚立蓉 张宏 司二静[1,2] 杨轲[1,2] 孟亚雄[1,2] 李葆春[1,3] 马小乐[1,2] 王化俊[1,2] LU Wen-Jia;WANG Jun-Cheng;YAO Li-Rong;ZHANG Hong;SI Er-Jing;YANG Ke;MENG Ya-Xiong;LI Bao-Chun;MA Xiao-Le;WANG Hua-Jun(State Key Laboratory of Arid Habitat Crop Science/Gansu Provincial Key Laboratory of Crop Genetic Improvement and Germplasm Innovation,Lanzhou 730070,Gansu,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,Gansu,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]省部共建干旱生境作物学国家重点实验室/甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《作物学报》2025年第5期1198-1214,共17页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05-02A-02);东西部科技协作专项(25CXNA030);国家重点实验室开放基金项目(GSCS-2021-02);甘肃省现代农业产业技术体系(2025);甘肃农业大学公招博士科研启动项目(GAU-KYQD-2022-10);甘肃农业大学伏羲青年英才计划(GAUfx-04Y011,Gaufx-03Y06);甘肃省教育厅产业支撑计划项目(2021CYZC-12);甘肃省陇原青年英才项目(2023)资助。

摘　　要：过氧化物酶(Class Ⅲ peroxidase, PRX)基因家族在调控植物生长发育及非生物胁迫过程中发挥重要作用。大麦(Hordeum vulgare L.)是典型的C3植物,目前关于HvPRXs基因家族的功能研究鲜有报道。本研究通过生物信息学的方法对大麦PRX基因家族(HvPRXs)成员进行了分析,探究其在20%PEG-6000胁迫下的表达模式。大麦全基因组中共鉴定出了178个HvPRXs基因家族成员,并根据其在染色体上位置顺序依次命名为HvPRX1~HvPRX178。通过对大麦、水稻和拟南芥的过氧化物酶进行进化树分析将其分为5个亚家族,基因结构和Domain分析表明同一亚家族具有较高的保守性。基因复制分析显示, 15个HvPRX基因(8%)存在片段复制, 34个HvPRX基因(67%)存在串联复制,串联复制事件在HvPRX基因扩增中起重要作用。大麦与拟南芥的物种间共线性分析显示,大麦与拟南芥有4个PRX直系同源基因对,说明基因从单子叶到双子叶进行了大规模的分子进化事件。转录组分析显示, HvPRXs基因家族成员在大麦根、叶中表达存在差异。启动子顺式作用元件分析显示,99个HvPRXs含有与干旱胁迫响应相关的顺式作用元件。最后基于qRT-PCR分析其干旱胁迫下的表达特征,结果表明,HvPRX1、HvPRX18、HvPRX63、HvPRX160和HvPRX167受20%PEG-6000诱导在3 h表达量显著升高。本研究结果为全面探索HvPRXs基因的生物学功能、其调控大麦抗旱性的分子机制以及抗逆作物新品种的培育提供参考。The Class III peroxidase(PRX)gene family plays a crucial role in regulating plant growth,development,and responses to abiotic stress.Barley(Hordeum vulgare L.),a typical C3 plant,has been relatively underexplored regarding the functional characterization of its HvPRX gene family.In this study,we performed a comprehensive analysis of HvPRX genes using bioin-formatics tools and investigated their expression patterns under drought stress induced by 20%PEG-6000 treatment.A total of 178 HvPRX gene family members were identified in the barley genome and were named HvPRX1–HvPRX178 based on their chromosomal positions.Phylogenetic analysis grouped the peroxidases of barley,rice,and Arabidopsis into five subfamilies,in-dicating evolutionary conservation.Gene structure and domain analyses revealed high conservation within the same subfamilies.Gene duplication analysis showed that 15 HvPRX genes(8%)underwent segmental duplication,while 34 HvPRX genes(67%)arose from tandem duplication,highlighting the critical role of tandem duplication events in HvPRX gene expansion.Interspecies collinearity analysis between barley and Arabidopsis identified four direct orthologous PRX gene pairs,suggesting that large-scale molecular evolution events occurred during the divergence from monocotyledons to dicotyledons.Transcriptome analysis demon-strated that HvPRX gene expression patterns varied between barley roots and leaves.Promoter analysis revealed that 99 HvPRX genes contained cis-acting elements associated with drought stress responses.Finally,qRT-PCR analysis was used to validate the expression profiles of HvPRX genes under drought stress.The expression levels of HvPRX1,HvPRX18,HvPRX63,HvPRX160,and HvPRX167 were significantly upregulated three hours after treatment with 20%PEG-6000.These findings provide valuable insights into the biological functions and molecular mechanisms of HvPRX genes in barley’s drought resistance.This study also lays a foundation for breeding stress-tolerant crop varieties.

关 键 词：大麦 PRX基因家族 生信分析 干旱胁迫 

分 类 号：S512.3[农业科学—作物学]