低浓度鱼藤素抑制活化T细胞增殖作用研究  

Inhibitory Effect of Low Concentrations of Deguelin on Activated T Cell Proliferation

作　　者：赵克天 敬林 崔文杰 张佳庆 伍佳丽 何晶晶 赖翼[5] 刘阳 罗兴燕 ZHAO Ketian;JING Lin;CUI Wenjie;ZHANG Jiaqing;WU Jiali;HE Jingjing;LAI Yi;LIU Yang;LUO Xingyan(Center for Scientific Research,Chengdu Medical College,Chengdu 610500,China;Guangxi Key Laboratory of Special Biomedicine,School of Medicine,Guangxi University,Nanning 530004,China;School of Pharmacy,Chengdu Medical College,Chengdu 610500,China;School of Clinical Medicine,Chengdu Medical College,Chengdu 610500,China;School of Laboratory Medicine,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院科研实验中心,成都610500 [2]广西大学医学院特色生物医药重点实验室,南宁530004 [3]成都医学院药学院,成都610500 [4]成都医学院临床医学院,成都610500 [5]成都医学院检验医学院,成都610500

出　　处：《中国药学杂志》2025年第5期515-521,共7页Chinese Pharmaceutical Journal

基　　金：国家级大学生创新项目资助(202217305005);四川省科技厅应用基础面上项目资助(2023NSFSC0624)。

摘　　要：目的探究低浓度鱼藤素抑制活化T细胞增殖的作用特点。方法Ficoll-Hypaque密度梯度离心法分离人外周血单个核细胞,免疫磁珠法纯化人T细胞,抗人CD3/CD28抗体活化T细胞。流式细胞术测定T细胞存活率、增殖指数、凋亡进展、CD25表达水平及分裂期比例;ELISA检测细胞因子IL-2、IL-4、IL-6、IL-17及IFN-γ的分泌水平。结果低浓度鱼藤素抑制抗人CD3/CD28抗体活化的T细胞增殖,IC_(50)为(73±12)nmol·L^(-1),400 nmol·L^(-1)时无细胞毒性。低浓度鱼藤素不影响活化的T细胞表达CD25和分泌IL-2,但提高G0/G1期细胞比例。低浓度鱼藤素促进活化T细胞分泌抗炎细胞因子IL-4,并抑制促炎细胞因子IL-6、IL-17及IFN-γ的产生。结论低浓度鱼藤素显著抑制T细胞增殖于G_(0)/G_(1)期,并有效抑制促炎细胞因子分泌,提示鱼藤素在自身免疫病的治疗中具有潜在作用。OBJECTIVE To explore the characteristics of the inhibitory effect of low concentrations of deguelin on the proliferation of activated T cells.METHODS Human peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation,human T cells were purified using immunomagnetic beads,and T cells were activated with Anti-CD3/CD28 antibodies.Flow cytometry was used to detect T cell survival rate,proliferation index,apoptosis progression,CD25 expression level,and cell division ratio;ELISA was used to detect cytokines IL-2,IL-4,IL-6,IL-17,and IFN-γsecretion levels.RESULTS Low concentrations of deguelin inhibit T cell proliferation activated by anti-human CD3/CD28 antibodies,with an IC_(50) of(73±12)nmol·L^(-1),and a concentration of 400 nmol·L^(-1)has no cytotoxicity.Low concentrations of deguelin do not affect the expression of CD25 and secretion of IL-2 in activated T cells but increases the proportion of G0/G1 phase cells.Low concentrations of deguelin promote the secretion of anti-inflammatory cytokine IL-4 and inhibit the production of pro-inflammatory cytokines IL-6,IL-17,and IFN-γin T cells.CONCLUSION Low concentrations of deguelin significantly inhibit T cell proliferation in the G_(0)/G_(1) phase and effectively suppress the secretion of pro-inflammatory cytokines,suggesting its potential role in the treatment of autoimmune diseases.

关 键 词：鱼藤素 T细胞 增殖抑制 低浓度 抗炎作用 细胞因子 

分 类 号：R282[医药卫生—中药学]