药用植物雷公藤JAZ基因家族的全基因组鉴定及功能分析  

Genome-wide identification and functional analysis of the JAZ gene family in Tripterygium wilfordii

作　　者：王柯燕 唐丽媚 吴林捷 王雨婷 陈夏唯 屠李婵 WANG Ke-yan;TANG Li-mei;WU Lin-jie;WANG Yu-ting;CHEN Xia-wei;TU Li-chan(School of Medicine,Hangzhou City University,Hangzhou 310015,China)

机构地区：[1]浙大城市学院医学院,浙江杭州310015

出　　处：《药学学报》2025年第4期1156-1165,共10页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82204559);浙江省自然科学基金资助项目(LQ23H280002)。

摘　　要：JAZ蛋白作为茉莉酸途径的抑制因子,对植物生长发育、植物次级代谢产物合成的调控具有重要作用。为研究雷公藤(Tripterygium wilfordii) JAZ基因家族的功能,本研究系统鉴定了雷公藤JAZ基因家族成员,分析其理化性质、基因染色体定位、系统进化树、蛋白质保守基序,同时通过雷公藤转录组测序数据分析TwJAZs基因在不同部位的表达模式,构建基因网络调控图谱,最终基因克隆获得18条TwJAZs基因的全长序列。结果表明,18个TwJAZs基因不均匀地分布在12条染色体上,其编码的氨基酸数量为122~525个,分子质量为13.87~54.73 kDa,等电点为6.83~10.27。亚细胞预测显示13个TwJAZs蛋白定位在细胞核上,5个位于细胞质和叶绿体上。系统进化树结果表明雷公藤JAZ家族蛋白可分为5个亚家族,同一亚家族成员具有相似的保守基序。表达模式分析结果表明TwJAZs基因主要在花、叶、去皮茎中具有较高的表达,且大部分TwJAZs基因在茉莉酸甲酯(MeJA)诱导4 h后的表达量最高。转录因子调控网络显示TwJAZs主要与AP2/ERF-ERF、NAC、bHLH等转录因子家族呈现强相关。本研究全面鉴定获得雷公藤中所有的JAZ基因家族序列,初步明确了TwJAZs基因家族的结构和功能特点,为深入研究TwJAZs蛋白功能和调控机制奠定重要基础。JAZ proteins,as inhibitors in the jasmonic acid(JA) signaling pathway,play a crucial role in regulating plant growth and development,as well as the biosynthesis of secondary metabolites in plants.To explore the functions of the JAZ gene family in Tripterygium wilfordii,our study systematically identified the members of the JAZ gene family in T.wilfordii.We analyzed their physicochemical properties,chromosome localization,phylogenetic tree,and protein conserved motifs.Additionally,we investigated the expression patterns of TwJAZs in different tissues,constructed a gene regulatory network map,and ultimately cloned the full-length sequences of 18JAZ genes.The results showed that the 18 TwJAZs were unevenly distributed across 12 chromosomes,encoding amino acid numbers ranging from 122 to 525,with molecular weights of 13.87 to 54.73 kDa,and isoelectric points ranging from 6.83 to 10.27.Subcellular localization prediction indicated that 13 TwJAZs were localized in the nucleus,while 5 TwJAZs were located in the cytoplasm and chloroplasts.The phylogenetic tree analysis revealed that the JAZ family proteins of T.wilfordii could be divided into five subfamilies,with members of the same subfamily sharing similar conserved motifs.Expression pattern analysis demonstrated that TwJAZs were predominantly expressed in flowers,leaves,and peeled stems,with most TwJAZs showing the highest expression levels after 4 hours of methyl jasmonate(MeJA) induction.The transcription factor regulatory network shows that TwJAZs are strongly correlated with transcription factor families such as AP2/ERF-ERF,NAC,and bHLH.This study comprehensively identified all the JAZ gene family sequences in T.wilfordii,initially clarifying the structural and functional characteristics of the TwJAZs,and laying an important foundation for further research on the functions and regulatory mechanisms of TwJAZs.

关 键 词：雷公藤 JAZ 生物信息学分析 表达模式 基因克隆 

分 类 号：R931[医药卫生—生药学]