面向体外诊断的体液蛋白质组学分析前因素研究进展  

Advances in the analysis of pre-analytical factors for in vitro diagnostic-oriented proteomics of body fluids

作　　者：李玙 应超 李奇萌 赵立芳 蔡燕宁 LI Yu;YING Chao;LI Qi-Meng;ZHAO Li-Fang;CAI Yan-Ning(Neurobiology Laboratory,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;Key Laboratory of Neurodegenerative Diseases,Ministry of Education,Beijing 100053,China;National Clinical Research Center for Geriatric Diseases,Beijing 100053,China;Clinical Sample Center,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院神经生物学研究室,北京100053 [2]教育部神经变性病重点实验室,北京100053 [3]国家老年疾病临床医学研究中心,北京100053 [4]首都医科大学宣武医院临床样本中心,北京100053

出　　处：《生命科学》2025年第3期321-328,共8页Chinese Bulletin of Life Sciences

基　　金：科技创新2030—重大项目(2021ZD0201100)。

摘　　要：在体外诊断领域,蛋白质组学研究对于生物标志物的发现、验证和确认至关重要。越来越多证据表明,不同的样本处理和操作步骤会影响蛋白质的丰度和检测的可靠性。体外诊断中的大多数异常和错误都发生在样本的分析前阶段,这些变量会对分析物的测定结果产生巨大影响。然而,目前关于分析前变量对样品采集和储存后蛋白质的稳定性以及完整性的影响尚不完全清楚。本文系统综述了分析前变量对生物体液蛋白质组学研究的影响,以期为蛋白质组学研究提供新的思路。In the domain of in vitro diagnostics,proteomics research plays a crucial role in the discovery,validation,and confirmation of biomarkers.Increasing evidence suggests that various sample handling and manipulation procedures significantly influence protein abundance and the reliability of assays.The majority of abnormalities and errors in in vitro diagnostics are observed during the pre-analytical phase of sample processing,and these variables can significantly influence the outcomes of the analyte assay.Nevertheless,the influence of pre-analytical variables on the stability and integrity of proteins following sample collection and storage remains inadequately comprehended.In this article,we systematically reviewed and analyzed the impact of pre-analytical variables on proteomics research of body fluids,aiming to provide new insights for proteomics research.

关 键 词：蛋白质组学 分析前因素 血液 脑脊液 尿液 

分 类 号：R331.5[医药卫生—人体生理学]