离子色谱-积分安培法测定水中碘化物的方法研究  

A method for determination of iodide in water by ion chromatography-integrated amperometric detection

作　　者：王秀莉[1] 于雪荣[1] 胡松 谢继安[1] 丁刚[3] 李卫东[3] 徐署东 Wang Xiuli;Yu Xuerong;Hu Song;Xie Ji'an;Ding Gang;Li Weidong;Xu Shudong(Physical and Chemical Laboratory of Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,China;Thermo Fisher Scientific(China)Co.,Ltd,Shanghai 201206,China;Office of the Center,Anhui Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]安徽省疾病预防控制中心理化检验室,合肥230601 [2]赛默飞世尔(中国)有限公司,上海201206 [3]安徽省疾病预防控制中心办公室,合肥230601

出　　处：《中华地方病学杂志》2025年第4期327-331,共5页Chinese Journal of Endemiology

摘　　要：目的建立水中碘化物的离子色谱-积分安培检测方法。方法水样经滤膜过滤后,采用离子色谱法的AS 11-HC阴离子色谱柱在70 mmol/L氢氧化钠溶液为淋洗液、进样量为100μl、柱温为30℃、流速为1.0 ml/min的条件下分离碘离子,并经银工作电极-积分安培检测法测定。在优化的实验条件下,开展校正曲线、检出限、定量限、精密度、准确度等方法学验证实验。结果碘化物在0~100μg/L浓度范围内符合平方校正曲线关系,相关系数(r)>0.9999;方法检出限为0.30μg/L,定量限为1.00μg/L。水中碘成分分析标准物质GBW09113f、GBW09114f的测定结果均在参考范围[(8.4±1.2)、(55±6)μg/L]。低本底水样的低、中、高3个浓度加标回收率范围为91.7%~97.2%,相对标准偏差范围为0.40%~1.60%。结论该方法具有水样前处理简便、灵敏度高、准确性好等特点,能够满足碘缺乏病监测中批量水样的微量碘化物测定。ObjectiveTo establish an ion chromatography-integrated amperometric detection method for iodide in water.MethodsAfter the water sample was filtered through a filter membrane,the AS 11-HC anion chromatography column of ion chromatography method was used to separate iodide ions under the conditions of 70 mmol/L sodium hydroxide solution as the eluent,injection volume of 100μl,column temperature of 30℃,and flow rate of 1.0 ml/min.The results were determined by silver working electrode integral amperometric detection method.Under the optimized experimental conditions,methodological evaluations such as method calibration curves,detection limits,quantification limits,precision,and accuracy were conducted.ResultsIodide followed a square correction curve within the concentration range of 0-100μg/L,with a correlation coefficient(r)>0.9999.The detection limit of the method was 0.30μg/L,and the quantification limit was 1.00μg/L.The determination results of the national standard substances GBW09113f and GBW09114f for iodine composition analysis in water were within the reference range[(8.4±1.2),(55±6)μg/L].The recovery rates of low,medium,and high concentration spiked samples with low background values ranged from 91.7%to 97.2%,and the relative standard deviation ranged from 0.40%to 1.60%.ConclusionThis method has the characteristics of simple water sample pretreatment,high sensitivity,and good accuracy,which can meet the determination of trace iodides in bulk water samples for iodine deficiency disorders monitoring.

关 键 词：碘化物 离子色谱 积分安培 微量 

分 类 号：R123.1[医药卫生—环境卫生学]