乌梅可改善咪喹莫特诱导的小鼠银屑病样皮炎  

Mume Fructus improves imiquimod-induced psoriasis-like dermatitis in mice

作　　者：汪晴 张红玉 张骏鸿 苏浩杰 刘凡露 周攀宇 卢月 吴晶晶 危建安 黎莉 韩凌 WANG Qing;ZHANG Hongyu;ZHANG Junhong;SU Haojie;LIU Fanlu;ZHOU Panyu;LU Yue;WU Jingjing;WEI Jianan;LI Li;HAN Ling(Chongqing Traditional Chinese Medicine Hospital,Chongqing 400011,China;Second Clinical College,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Guangdong Academy of Traditional Chinese Medicine,Research Team of Molecular Biology and Systems Biology of Chinese medicine,Guangzhou 510006,China;Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome,Guangzhou 510006,China;Guangdong-HongKong-Macao Joint Lab on Chinese Medicine and Immune Disease Research,Guangzhou 510006,China;Chinese Medicine Guangdong Laboratory,Zhuhai 519000,China)

机构地区：[1]重庆市中医院,重庆400011 [2]广州中医药大学第二临床医学院,广东广州510006 [3]广东省中医药科学院中医药分子生物与系统生物学团队,广东广州510006 [4]广东省中医证候临床研究重点实验室,广东广州510006 [5]粤港澳中医药与免疫疾病研究联合实验室,广东广州510006 [6]中医药广东省实验室,广东珠海519000

出　　处：《皮肤性病诊疗学杂志》2025年第3期166-175,共10页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金(82374313);国家自然科学基金(82305257);广东省自然科学基金项目(2020A1515010607);广东省科技计划项目(2020B1111100006);广州市科技计划项目(202201020332);省部共建中医湿证国家重点实验室重点项目(SZ2021ZZ29);广东省中医院拔尖人才科研专项资助(BJ2022KY05)。

摘　　要：目的探索乌梅对咪喹莫特(IMQ)诱导的小鼠银屑病样皮损的干预作用及潜在机制。方法将36只BALB/c雄性小鼠随机分为6组:正常组,模型组,地塞米松组,乌梅低、中、高剂量组,每组6只。各组小鼠预防性给药3 d,地塞米松组,乌梅低、中、高剂量组小鼠分别给予地塞米松5 mg/(kg·d),乌梅0.3、0.6、1.2 g/(kg·d)灌胃,正常组及模型组给予饮用水灌胃。除正常组外,其余各组小鼠第4天开始每日上午在背部剃毛区涂抹IMQ乳膏制备银屑病样小鼠模型,每日下午各组继续按预防性给药剂量灌胃,连续7 d。造模期间,记录各组小鼠体质量变化、背部皮损严重程度评分(PASI)、背部皮肤经皮水分丢失量(TEWL)。HE染色观察小鼠皮损组织的病理变化;免疫组化检测皮损组织中CD3的表达;qRT-PCR检测IL-1β、IL-6、TNF-α、LOR、IVL的mRNA表达水平。结果与模型组相比,乌梅各剂量组小鼠背部红斑、鳞屑及皮损浸润增厚明显改善,PASI评分、TEWL、表皮厚度下降。免疫组化结果表明,乌梅各剂量组CD3表达水平下降,真皮层T细胞浸润减少。qRT-PCR结果表明,与模型组相比,乌梅低、中、高剂量组IL-1βmRNA表达水平明显降低(均P<0.05),低、中剂量组IL-6 mRNA表达水平明显降低(均P<0.01),中、高剂量组TNF-αmRNA表达水平明显降低(均P<0.01),乌梅中剂量组LOR(P<0.001)、IVL(P<0.05)mRNA表达水平明显上升。结论乌梅可改善咪喹莫特诱导的小鼠银屑病样皮炎,抑制炎症因子表达,保护小鼠皮肤屏障。Objective To investigate the therapeutic effects and potential mechanisms of Mume Fructus on imiquimod(IMQ)-induced psoriasis-like dermatitis in mice.Methods Thirty-six male BALB/c mice were randomly divided into six groups:i.e,the normal group,model group,dexamethasone-treated group,and Mume Fructus at low-dose,medium-dose,and high-dose groups.Mice,except those in the normal and model groups,were given dexamethasone at a daily dose of 5 mg/kg,or Mume Fructus at a daily dose of 0.3,0.6,or 1.2 g/kg,respectively,via gavage.The normal and model groups were given water via gavage.Except for the normal group,the other groups were treated topically with IMQ cream to the shaved dorsal area once daily from day 4 to day 7 to induce psoriasis-like dermatitis.During the induction of dermatitis,changes in body weight,psoriasis area and severity index(PASI)scores,and transepidermal water loss rates(TEWL)on the dorsal skin were measured.Hematoxylin-Eosin(HE)staining was used to observe the pathological changes in the skin lesion,and immunohistochemistry was performed to detect the expression of CD3 in the skin lesion.The expression levels of mRNA for IL-1β,IL-6,TNF-α,LOR,and IVL were detected by qRT-PCR.Results Compared with the model group,Mume Fructus improved the erythema,scaling,and inflammatory infiltration.Meanwhile,the PASI scores,TEWL,and epidermal thickness were significantly reduced.Immunohistochemistry indicated that Mume Fructus inhibited CD3 expression and reduced T-cell infiltration in the dermis.Moreover,Mume Fructus significantly lowered the expression levels of mRNA for IL-1β(P<0.05 vs.the model group for all doses),IL-6(P<0.01 vs.model group for low-and medium-doses),and TNF-α(P<0.01 vs.model group for medium and high doses),while increasing the expression levels of mRNA for LOR(P<0.001 vs.model group for medium-dose)and IVL(P<0.05 vs.model group for medium-dose).Conclusion Mume Fructus ameliorates imiquimod-induced psoriasis-like dermatitis,inhibits expression of inflammatory factors,and protects the ski

关 键 词：银屑病 乌梅 炎症 皮肤屏障 咪喹莫特 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]