Application of genome tagging technology in elucidating the function of sperm-specific protein 411(Ssp411)  

作　　者：Xue-Hai Zhou Min-Min Hua Jia-Nan Tang Bang-Guo Wu Xue-Mei Wang Chang-Gen Shi Yang Yang Jun Wu Bin Wu Bao-Li Zhang Yi-Si Sun Tian-Cheng Zhang Hui-Juan ShiShanghai-MOST Key Laboratory of Health and Disease Genomics NHC Key Lab of Reproduction Regulation Shanghai Institute for Biomedical and Pharmaceutical 

机构地区：[1]Technologies,School of Pharmacy,Fudan University,Shanghai 200237,China

出　　处：《Asian Journal of Andrology》2025年第1期120-128,共9页亚洲男性学杂志(英文版)

基　　金：the support from the National Natural Science Foundation of China(No.32070849);The Foundation of Science and Technology Commission of Shanghai Municipality(No.22DX1900400);Science and Technology Commission of Shanghai Municipality(No.23JC1403803);Shanghai Municipal Science and Technology Commission Targeted Funding Project(No.22DX1900400).

摘　　要：The genome tagging project(GTP)plays a pivotal role in addressing a critical gap in the understanding of protein functions.Within this framework,we successfully generated a human influenza hemagglutinin-tagged sperm-specific protein 411(HA-tagged Ssp411)mouse model.This model is instrumental in probing the expression and function of Ssp411.Our research revealed that Ssp411 is expressed in the round spermatids,elongating spermatids,elongated spermatids,and epididymal spermatozoa.The comprehensive examination of the distribution of Ssp411 in these germ cells offers new perspectives on its involvement in spermiogenesis.Nevertheless,rigorous further inquiry is imperative to elucidate the precise mechanistic underpinnings of these functions.Ssp411 is not detectable in metaphase Ⅱ(MⅡ)oocytes,zygotes,or 2-cell stage embryos,highlighting its intricate role in early embryonic development.These findings not only advance our understanding of the role of Ssp411 in reproductive physiology but also significantly contribute to the overarching goals of the GTP,fostering groundbreaking advancements in the f ields of spermiogenesis and reproductive biology.

关 键 词：epididymal spermatozoa genome tagging project HA tag sperm-specific protein 411 

分 类 号：Q78[生物学—分子生物学]