ICAM1在电针治疗LPS诱导急性肺损伤大鼠中的作用机制  

The mechanism of ICAM1 in the treatment of LPS induced acute lung injury in rats with electroacupuncture

作　　者：钟培瑞 廖瑛 伍琦 孙光华 刘静 周君 陈佳倩 谭金曲 赖桂花 曾亚华 屈萌艰 何晓艳 ZHONG Pei-Rui;LIAO Ying;WU Qi(Rehabilitation Medicine Center,the First Affiliated Hospital of Hengyang Medical College,University of South China,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学衡阳医学院附属第一医院康复医学中心,湖南衡阳421001 [2]南华大学衡阳医学院附属第一医院康复医学科,湖南衡阳421001 [3]南华大学衡阳医学院附属第一医院康复医学实验室,湖南衡阳421001 [4]衡阳市第一人民医院呼吸内科

出　　处：《中国老年学杂志》2025年第8期1899-1903,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金面上项目(No.81973917);国家自然科学基金青年项目(No.82105022);湖南省卫健委项目(No.202203104147);湖南省自然科学基金(No.2021JJ40507)。

摘　　要：目的 研究细胞间黏附分子(ICAM)-1在电针治疗脂多糖(LPS)诱导的SD大鼠急性肺损伤(ALI)模型中的作用机制。方法 3月龄雄性SD大鼠随机分为对照组、ALI组、电针治疗组,每组8只。通过尾静脉缓慢注射LPS(5 mg/kg)诱导肺损伤模型,对照组注射等体积生理盐水。电针治疗组于造模后即进行干预(穴位选择:“足三里”“尺泽”。干预参数:波形疏密波,疏波3 Hz,密波15 Hz,电流强度1 mA),1次/d, 30 min/次,连续5 d。干预5 d后,处死各组。肺组织行苏木素-伊红(HE)染色、半定量评分及湿/干重比(W/D);酶联免疫吸附试验(ELISA)法检测血清炎性因子[白细胞介素(IL)-6、IL-18];实时荧光定量逆转录聚合酶链反应(RT-qPCR)和Western印迹检测ICAM1 mRNA和蛋白表达。结果 ALI组肺组织损伤严重,可见肺组织内炎性细胞浸润、间质水肿、广泛肺泡损伤等,而电针治疗能有效改善上述病理表现,显著降低肺组织损伤评分(P<0.01)。ALI组W/D、IL-6、IL-18水平、ICAM1 mRNA及蛋白表达显著高于对照组(P<0.01);电针可显著降低大鼠肺损伤后W/D、IL-6、IL-18、ICAM1 mRNA及蛋白表达(P<0.01,P<0.05)。结论 LPS能够激活肺部炎症反应、增加肺部渗出、诱导ALI,其机制之一可能是通过上调ICAM1激发炎症反应。电针可能通过抑制ICAM1表达来缓解炎症反应,减轻肺部损伤。ICAM1可能为ALI治疗的潜在作用靶点。Objective To study the mechanism of intercellular adhesion molecule(ICAM)-1 in the treatment of lipopolysaccharide(LPS)induced acute lung injury(ALI)in SD rat model with electroacupuncture.Methods Three month old male SD rats were randomly divided into control group,ALI group and electroacupuncture treatment group,with 8 rats in each group.Lung injury model was inducted by slow injection of LPS(5 mg/kg)via tail vein,while the control group was injected with an equal volume of physiological saline.The electroacupuncture treatment group was given intervention immediately after modeling(acupoint selection:Zusanli"and"Chize".Intervention parameters:sparse wave,sparse wave 3 Hz,dense wave 15 Hz,current intensity ImA),once a day,30 min each time,continuous treatment for 5 d.After 5 d of intervention,all groups were euthanized.Hematoxylin-eosin(HE)staining,semi quantitative scoring,and wet/dry weight ratio(W/D)were performed on lung tissues;enzyme linked immunosorbent assay(ELISA)method was used to detect serum inflammatory factors[interleukin(IL)-6,IL-18];real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-qPCR)and Western blot were used to detect the mRNA and protein of ICAM1.Results The ALI group showed severe lung tissue damage,presenting pathological manifestations such as inflammatory cell infiltration,interstitial edema and diffuse alveolar injury in lung tissues.The above pathological manifestations were effectively improved and the lung tissue injury score was significantly reduced by electroacupuncture treatment(P<0.01).The levels of W/D,IL-6,IL-18 and the mRNA and protein of ICAMI in the ALI group were significantly higher than those in the control group(P<0.O1).The expression levels of W/D,IL-6,IL-18,the mRNA and protein expressions of ICAMI in rats with lung injury were significantly reduced by electroacupuncture(P<0.01,P<0.05).Conclusions LPS could activate pulmonary inflammatory response,increase pulmonary exudation,and induce ALI.One of its mechanisms may be to stimulate

关 键 词：急性肺损伤 电针 细胞间黏附分子(ICAM)-1 

分 类 号：R245.97[医药卫生—针灸推拿学]