滇牡丹花瓣色斑形成的分子机制研究  

Molecular mechanism of the colour spot formation in Paeonia delavayi petals

作　　者：李海清 杜春 王娟 张怀璧 LI Haiqing;DU Chun;WANG Juan;ZHANG Huaibi(College of Landscape and Horticulture,Southwest Forestry University,Kunming 650224,China;College of Forestry,Southwest Forestry University,Kunming 650224,China;New Zealand Institute for Plant&Food Research Limited,Palmerston North 11600,New Zealand)

机构地区：[1]西南林业大学园林园艺学院,中国昆明650224 [2]西南林业大学林学院,中国昆明650224 [3]新西兰皇家植物与食品研究所,新西兰北帕默斯顿11600

出　　处：《广西植物》2025年第4期628-640,共13页Guihaia

基　　金：云南省重大基础专项生物资源数字化开发应用项目(202002AA100007);国家自然科学基金(32060089);云南省万人计划“云岭产业技术领军人才”专项资助项目(云发改[2018]212号)。

摘　　要：为探究滇牡丹花瓣色斑形成的分子机制,该研究以滇牡丹有色斑和无色斑的黄色花瓣为试验材料,利用Illumina平台进行转录组测序和UPLC系统进行代谢组分析,筛选影响滇牡丹色斑生成的关键差异表达基因和转录因子。结果表明:(1)转录组测序共获得63981条Unigenes,平均长度为805 bp,注释到的Unigenes占比68.24%。筛选到19496个差异表达基因,其中有41个差异表达基因参与类黄酮生物合成,筛选出差异显著的DFR、CHS和CHI 3条结构基因。在得到的37个MYB转录因子中,筛选到1个R2R3-MYB转录因子PdMYB 30在促进色斑生成中发挥了重要作用。(2)通过UPLC-MS/MS平台靶向测定检测到44种花青素化合物。(3)通过验证RNA-seq中差异表达基因的表达变化趋势与qRT-PCR结果一致。综上表明,滇牡丹色斑的形成主要受花青素的影响,转录因子PdMYB 30与结构基因CHS、CHI和DFR在红色斑黄花硬蕾期(B-S1)因相似的高表达而呈正相关,预测PdMYB 30可能是在类黄酮生物合成中促进色斑形成的正调节因子,可以提高类黄酮生物合成通路中结构基因的表达量,从而促进植物中花青素的积累。该研究为制定高效的滇牡丹育种技术提供了科学依据。To investigate the molecular mechanisms of colour spot formation in Paeonia delavayi petals,this study used yellow petals of P.delavayi with and without colour spot as experimental material.Utilizing the Illumina platform for transcriptome sequencing and the UPLC system for metabolome analysis,the key differentially expressed genes(DEGs)and transcription factors affecting colour spot formation in P.delavayi were screened.The results were as follows:(1)The transcriptome sequencing yielded 63981 Unigenes with an average length of 805 bp,and 68.24%of these Unigenes were annotated.A total of 19496 DEGs were identified,of which 41 DEGs were involved in flavonoid biosynthesis and DFR,CHS,and CHI structural genes showed significant differential expression.Among the obtained 37 MYB transcription factors,one R2R3-MYB transcription factor,PdMYB 30,was found to play a significant role in promoting colour spot formation.(2)Targeted analysis using the UPLC-MS/MS platform detected 44 anthocyanin compounds.(3)The expression trends of DEGs identified by RNA-seq were consistent with the qRT-PCR results.In summary,the colour spot formation of P.delavayi is mainly influenced by anthocyanins.The transcription factor PdMYB 30 is positively correlated with the structural genes CHS,CHI,and DFR during the yellow flower with red spot bud stage(B-S1)due to their similar high expression levels.It is predicted that PdMYB 30 may act as a positive regulator in the flavonoid biosynthesis pathway,enhancing the expression levels of structural genes involved in flavonoid biosynthesis,thereby promoting the accumulation of anthocyanins in the plant.This study provides a scientific basis for developing efficient breeding techniques for P.delavayi.

关 键 词：滇牡丹 色斑形成 转录组 代谢组 差异表达基因 

分 类 号：Q943[生物学—植物学]