天贵卷瓣兰组培快繁技术研究  

Tissue culture and rapid propagation of Bulbophyllum tianguii

作　　者：王雅珂 吴巧芬 柴胜丰[2] 杨燕妮 刘巧 郑文俊 蒋强 邓振海 仇硕[2] WANG Yake;WU Qiaofen;CHAI Shengfeng;YANG Yanni;LIU Qiao;ZHENG Wenjun;JIANG Qiang;DENG Zhenhai;QIU Shuo(College of Tourism&Landscape Architecture,Guilin University of Technology,Guilin 541006,Guangxi,China;Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,Guangxi,China;Guangxi Yachang Orchid National Nature Reserve Management Center,Baise 533209,Guangxi,China)

机构地区：[1]桂林理工大学旅游与风景园林学院,广西桂林541006 [2]广西壮族自治区中国科学院广西植物研究所,广西植物功能物质与资源持续利用重点实验室,广西桂林541006 [3]广西雅长兰科植物国家级自然保护区管理中心,广西百色533209

出　　处：《广西植物》2025年第4期678-688,共11页Guihaia

基　　金：国家重点研发计划课题(2022YFF1300703);广西重点研发计划项目(桂科AB24010138);广西林业科技推广示范项目(桂林科字[2021]第28号);广西植物研究所基本业务费项目(桂植业23011);。

摘　　要：为了建立天贵卷瓣兰组培快繁技术体系,该研究以种子为外植体,筛选适宜其种子萌发及丛生芽分化、增殖、生根的培养基和生根苗移栽驯化的栽培基质。结果表明:(1)天贵卷瓣兰种子萌发的最适培养基为MS+6-BA 2.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)+椰汁200 mL·L^(-1)+蔗糖30 g·L^(-1)+琼脂7 g·L^(-1)。(2)丛生芽分化的最适培养基为B 5+IBA 0.2 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂7 g·L^(-1)。(3)丛生芽增殖的最佳培养基为MS+6-BA 2.0 mg·L^(-1)+KT 0.1 mg·L^(-1)+NAA 0.1 mg·L^(-1)+椰汁200 mL·L^(-1)+蔗糖30 g·L^(-1)+活性炭1 g·L^(-1)+琼脂7 g·L^(-1),增殖系数达6.70。(4)丛生芽生根的最佳培养基为MS+6-BA 1.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+KT 0.5 mg·L^(-1)+椰汁200 mL·L^(-1)+蔗糖30 g·L^(-1)+活性炭1 g·L^(-1)+琼脂7 g·L^(-1),生根率、生根数以及根长分别达100%、6.19、3.15 cm。(5)生根苗在蛭石和珍珠岩体积比2∶1的移栽基质中成活率最高,达100%,发育良好。该研究为天贵卷瓣兰组培快繁和规模化生产奠定了基础,有利于其资源保护。In order to establish the tissue culture and rapid propagation of Bulbophyllum tianguii,the seeds of B.tianguii were selected as explants to screen for suitable culture media for promoting seed germination,cluster bud differentiation,proliferation,rooting,and the growing media for transplanting and domesticating of rooted seedlings.The results were as follows:(1)The most optimal medium for seed germination of B.tianguii was MS+6-BA 2.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)+coconut milk 200 mL·L^(-1)+sucrose 30 g·L^(-1)+agar 7 g·L^(-1).(2)The optimal culture medium for cluster bud differentiation was B 5+IBA 0.2 mg·L^(-1)+sucrose 30 g·L^(-1)+agar 7 g·L^(-1).(3)The optimal medium for cluster bud proliferation was MS+6-BA 2.0 mg·L^(-1)+KT 0.1 mg·L^(-1)+NAA 0.1 mg·L^(-1)+coconut milk 200 mL·L^(-1)+sucrose 30 g·L^(-1)+activated carbon 1 g·L^(-1)+agar 7 g·L^(-1).The proliferation coefficient reached a high value of 6.70.(4)The optimal medium for cluster bud rooting was MS+6-BA 1.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+KT 0.5 mg·L^(-1)+coconut milk 200 mL·L^(-1)+sucrose 30 g·L^(-1)+activated carbon 1 g·L^(-1)+agar 7 g·L^(-1).The rooting rate,rooting number and root length reached 100%,6.19 and 3.15 cm,respectively.(5)The survival rate of rooted seedlings reached 100%when transplanted into media(vermiculite∶perlite=2∶1).The study not only provides a foundation for the rapid propagation and large-scale production of B.tianguii,but also is beneficial to resource conservation.

关 键 词：天贵卷瓣兰 无菌播种 丛生芽分化 丛生芽增殖 移栽驯化 

分 类 号：Q943.1[生物学—植物学]