恒河猴生殖道Ⅲ型干扰素表达的初步研究  

Preliminary study of type Ⅲ interferon expression in the genital tract of rhesus macaques

作　　者：刘瑞洁 杨贵波[1] LIU Ruijie;YANG Guibo(National Center for AIDS/STD Control and Prevention,China-CDC,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京102206

出　　处：《中国实验动物学报》2025年第3期365-373,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81571607)。

摘　　要：目的 为了阐述恒河猴Ⅲ型干扰素(IFNL)系统的生物学特征,促进恒河猴在感染性疾病的病理、药物和疫苗研究中的应用,本文对恒河猴生殖道IFNL及IFNLR1的表达进行了研究。方法 建立基因特异的RT-PCR方法,对正常和SHIV/SIV感染过的恒河生殖道组织中IFNL1、IFNL3和IFNLR1 mRNA的表达进行观察,利用共聚焦显微术对IFNLR1的组织分布进行观察。结果 对正常恒河猴阴道和子宫组织的RT-PCR检测未见明确的IFNL1和IFNL3 mRNA扩增条带,但可明确检测到IFNLR1的mRNA和缺失第6个外显子的变体;IFNLR1阳性染色主要位于阴道黏膜上皮和子宫内膜被覆上皮及腺上皮中。在部分SHIV/SIV感染个体中,IFNL1和IFNL3的mRNA水平明显增高,而IFNLR1的mRNA水平几乎没有变化;但Mx1、Mx2和OAS等干扰素刺激基因的mRNA水平显著升高。结论 本研究数据表明恒河猴生殖道可表达IFNL1、IFNL3和IFNLR1,为利用恒河猴开展艾滋病等人类生殖道感染疾病研究提供了基础。Objective To determine the expression of type Ⅲ interferon(IFNL) and IFNL receptor 1(IFNLR1) in the genital tract in rhesus macaques to elucidate the biological characteristics of rhesus macaque IFNL system and promote the use of these monkeys in studies on the pathology, drugs and vaccines for human diseases. Methods IFNL1, IFNL3, and IFNLR1 mRNA levels in normal and simian-human immunodeficiency virus(SHIV)/simian immunodeficiency virus(SIV)-infected genital tract tissues of rhesus macaques were detected by gene-specific reverse transcription-polymerase chain reaction, and IFNLR1 immunoreactivity was examined by confocal microscopy. Results IFNL1 and IFNL3 mRNA levels in the uterus and vagina in normal rhesus macaques were below the measurement threshold, but mRNA levels of IFNLR1 and its variant lacking exon 6 could be readily detected. IFNLR1 immunoreactivity was primarily detected in the covering or glandular epithelia of the uterus and vagina. IFNL1 and IFNL3 mRNA levels were increased in some SHIV/SIV-infected macaques, while mRNA levels of IFNLR1 were unchanged and mRNA levels of IFN-stimulated genes(Mx1, Mx2, and OAS) were significantly increased. Conclusions These data indicate that the type Ⅲ IFN system is expressed in the reproductive tract of rhesus macaques, as in humans, thus providing a scientific basis for the use of rhesus macaques in studies of human infectious diseases, such as AIDS.

关 键 词：恒河猴 Ⅲ型干扰素 生殖道 免疫缺陷病毒感染 

分 类 号：Q95-33[生物学—动物学]