青钱柳对棕榈酸钠诱导的HepG2细胞脂质沉积的影响及作用机制  

Effects and Mechanism of Cyclocaryae Paliuri Folium in Sodium Palmitate-Induced Lipid Deposition in HepG2 Cells

作　　者：刘艺璇 吴昊洋 阳晶晶[1,2] 蔡昱哲 罗政 李定祥 邓奕辉 LIU Yixuan;WU Haoyang;YANG Jingjing;CAI Yuzhe;LUO Zheng;LI Dingxiang;DENG Yihui(Hunan Province Key Laboratory of Cerebrovascular Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;School of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;College of Acupuncture&Moxibustion and Tui-na Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学中西医结合心脑疾病防治湖南省重点实验室,湖南长沙410208 [2]湖南中医药大学中医学院,湖南长沙410208 [3]湖南中医药大学针灸推拿与康复学院,湖南长沙410208

出　　处：《中国中医药信息杂志》2025年第4期72-78,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点研发计划(2022YFC3501202);湖南省科技创新计划(2020RC4050);湖南省卫生健康委科研课题(D202303067824);长沙市自然基金(kq2208188);湖南省中医药科研课题(B2023057)。

摘　　要：目的探讨青钱柳对棕榈酸钠诱导的HepG2细胞脂质沉积的改善作用及潜在机制。方法通过CCK-8法测定棕榈酸钠、青钱柳冻干粉对HepG2细胞活力的影响,确定给药剂量。将细胞分为空白组、模型组、吡格列酮组和青钱柳低、中、高剂量组,采用350μmol/L棕榈酸钠诱导建立HepG2细胞脂质沉积模型,药物组分别予吡格列酮和低、中、高剂量青钱柳冻干粉(100、250、500μg/mL)干预12 h。采用尼罗红染色及BODIPY493/503荧光探针观察细胞内脂质沉积,ELISA检测细胞上清液肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6含量,Western blot检测磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(Akt)、胆固醇调节元件结合蛋白-1(SREBP-1)、脂肪酸合成酶(FAS)、Bcl-2、Bax蛋白表达,qPCR检测脂肪甘油三酯酶(ATGL)、CD36 mRNA表达。结果与空白组比较,模型组HepG2细胞有大量脂质沉积,细胞上清液TNF-α、IL-6含量显著升高(P<0.01),细胞p-PI3K、p-Akt、SREBP-1、FAS、Bax蛋白表达显著升高,Bcl-2蛋白表达显著降低(P<0.01),ATGL mRNA表达显著降低,CD36 mRNA表达显著升高(P<0.01);与模型组比较,吡格列酮组和青钱柳各剂量组细胞内脂质沉积不同程度改善,细胞上清液TNF-α、IL-6含量降低,细胞p-PI3K、p-Akt、SREBP-1、FAS、Bax蛋白表达降低,Bcl-2蛋白表达升高,ATGL mRNA表达升高,CD36 mRNA表达降低,其中吡格列酮组和青钱柳高剂量组差异有统计学意义(P<0.05,P<0.01)。结论青钱柳可能通过调控PI3K/Akt/SREBP-1/FAS信号通路影响三酰甘油代谢,改善棕榈酸钠诱导的HepG2模型细胞脂质沉积。Objective To investigate the ameliorative effect and potential mechanism of Cyclocaryae Paliuri Folium in sodium palmitate-induced lipid deposition in HepG2 cells.Methods The effect of sodium palmitate and lyophilized powder of Cyclocaryae Paliuri Folium on the viability of HepG2 cells was determined by the CCK-8 method to determine the subsequent dosage administered.The HepG2 cells were divided into blank group,model group,pioglitazone group and Cyclocaryae Paliuri Folium low-,medium-and high-dosage group,the lipid deposition model of HepG2 cells was established using 350μmol/L sodium palmitate,the medication group were given pioglitazone and low,medium-and high-dosage of Cyclocaryae Paliuri Folium(100,250,500μg/mL)for 12 h respectively.The intracellular lipid deposition was observed by Nile red staining and BODIPY493/503 fluorescent probe staining,the content of TNFαand IL-6 in supernatant of cell culture medium were detected by ELISA,Western blot was used to detect PI3K,Akt,sterol-regulatory element binding protein-1(SREBP-1),fatty acid synthase(FAS),Bcl-2,Bax protein expression,qPCR was used to detect the mRNA expression of adipose triglyceride lipase(ATGL)and CD36.Results Compared with the blank group,the lipid deposition of HepG2 cells in the model group increased,TNF-αand IL-6 contents in the supernatant of cell culture medium significantly increased(P<0.01),the protein expressions of p-PI3K,p-Akt,SREBP-1,FAS and Bax in cells significantly increased,while the protein expression of Bcl-2 significantly decreased(P<0.01),the mRNA expression of ATGL significantly decreased,and the mRNA expression of CD36 significantly increased(P<0.01).Compared with the model group,the intracellular lipid deposition of the pioglitazone group and Cyclocaryae Paliuri Folium groups improved to varying degrees,the contents of TNFαand IL-6 in the cell supernatant decreased,the expressions of p-PI3K,p-Akt,SREBP-1,FAS and Bax proteins decreased,the expression of Bcl-2 protein increased,the expression of ATGL mRNA increased,and t

关 键 词：青钱柳 HEPG2细胞 脂质沉积 PI3K/AKt/SREBP-1/FAS信号通路 

分 类 号：R285.5[医药卫生—中药学]