滋肾育胎丸对多囊卵巢综合征早期流产的调控及其机制  

Regulation and Mechanism of Zishen Yutai Pill(滋肾育胎丸)on Early Abortion with Polycystic Ovary Syndrome

作　　者：郭肖竹 吴登辉 张旭 秦臻[1] 仲丽丽[2] 张跃辉[2] GUO Xiaozhu;WU Denghui;ZHANG Xu;QIN Zhen;ZHONG Lili;ZHANG Yuehui(Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Wuxing District People's Hospital of Huzhou,Huzhou 313000,Zhejiang,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [3]湖州市吴兴区人民医院,浙江湖州313000

出　　处：《辽宁中医药大学学报》2025年第5期36-41,共6页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(82074259);罗元恺滋肾育胎丸中青年科研基金项目(20190804);黑龙江中医药大学研究生创新基金项目(2022yjscx005)。

摘　　要：目的观察滋肾育胎丸对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠早期流产的调控及调控机制。方法将27只雌性妊娠SD大鼠随机分为对照组、模型组及滋肾育胎丸组,每组9只。给药方式:对照组使用等体积0.9%氯化钠溶液和大豆油腹股沟皮下注射,并使用等体积0.9%氯化钠溶液灌胃,连续7 d;PCOS模型组使用双氢睾酮1.66 mg·kg^(-1)·d^(-1)(日1次)和胰岛素注射液6 IU/d(早晚各3 IU)腹股沟皮下注射,并使用等体积0.9%氯化钠溶液灌胃,连续7 d;滋肾育胎丸治疗组使用双氢睾酮1.66 mg·kg^(-1)·d^(-1)(日1次)和胰岛素注射液6 IU/d(早晚各3 IU)腹股沟皮下注射,滋肾育胎丸1.5 mg·kg^(-1)·d^(-1)(日1次)于0.9%氯化钠溶液溶解灌胃,连续7 d。实验步骤:记录每日体质量变化,在妊娠第7日行口服葡萄糖耐量试验;妊娠第8日晨取材,评估胚胎着床情况,记录胚胎着床数量、血胚(棕褐色)数量;获取血清、子宫、卵巢组织,通过酶联免疫吸附测定法(ELISA)测定血清雌二醇(E2)、孕酮(P)、睾酮(T)、性激素结合球蛋白(SHBG)和空腹胰岛素(FINS)水平;计算游离雄激素指数(FAI)、胰岛素抵抗指数(HOMA-IR)和葡萄糖曲线下面积(AUC);通过苏木精和伊红染色观察子宫的形态;实时荧光定量聚合酶链式反应(q RT-PCR)检测子宫葡萄糖转运蛋白4(GLUT4)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白血病抑制因子(LIF)、同源框A10(HOXA10)的m RNA表达;蛋白免疫印迹法(WB)测定大鼠子宫中磷脂酰肌醇-3激酶/蛋白激酶B(PI3K/Akt)信号通路关键分子的蛋白表达水平。结果与对照组相比,模型组大鼠胚胎着床数量降低,血胚数量明显增多,卵巢和子宫重量减轻,子宫内膜结构改变;血清T、FINS、FAI、HOMA-IR和AUC升高,E2和SHBG降低(P<0.05),糖耐量和胰岛素敏感性降低;大鼠子宫组织中IL-6、TNF-αm RNA表达水平均升高,p-PI3K和p-Akt表达水平明显降低,差异均具有统计学意�Objective To observe the regulation and mechanism of Zishen Yutai Pill(滋肾育胎丸)on early abortion in rats with polycystic ovary syndrome(PCOS).Methods Twenty-seven pregnant female SD rats were randomly divided into control group,model group and Zishen Yutai Pill group,with 9 rats in each group.Control group used the equal volume of saline and soybean oil(dihydrotestosterone solvent)which were injected subcutaneously in the groin,and the equal volume of saline was gavaged for 7 consecutive days.In PCOS model group,dihydrotestosterone(1.66 mg·kg^(-1)·d^(-1),once a day)plus insulin(6 IU/day,3 IU in the morning and 3 IU at night)were injected subcutaneously in the groin to induce PCOS-like hyperandrogenism and hyperinsulinaemia,and the equal volume of saline was gavaged for 7 consecutive days.In Zishen Yutai Pill group,dihydrotestosterone(1.66 mg·kg^(-1)·d^(-1),once a day)plus insulin(6 IU/day,3 IU in the morning and 3 IU at night)were injected subcutaneously in the groin to induce PCOS-like hyperandrogenism and hyperinsulinaemia,and weight changes were recorded,and oral glucose tolerance tests(OGTT)was performed on the 7th day of pregnancy.On the 8th day of pregnancy,embryo implantation was assessed,and the number of implanted embryos and haemorrhagic embryos(brown)was recorded.Serum,uterine and ovarian tissues were obtained,and the levels of estradiol(E2),progesterone(P),testosterone(T),sex hormone-binding globulin(SHBG)and fasting insulin(FINS)were measured by enzyme-linked immunosorbent assay(ELISA).Free androgen index(FAI),homeostatic assessment insulin resistance index(HOMA-IR)and area under the glucose curve(AUC)were calculated.The morphology of uterus was observed by hematoxylin and eosin staining.The mRNA expressions of glucose transporter 4(GLUT4),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),leukemia suppressor factor(LIF)and homeobox A10(HOXA10)were detected by qRT-PCR.The protein expression levels of key molecules of phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)signaling path

关 键 词：滋肾育胎丸 多囊卵巢综合征 早期流产 调控机制 

分 类 号：R271.1[医药卫生—中医妇科学]