子宫糖基化蛋白、可溶性酪氨酸激酶受体1水平与子痫前期严重程度及妊娠结局的相关性  

Correlation of glycodelin-A and serum soluble tyrosine kinase receptor 1 levels with the severity of preeclampsia and pregnancy outcomes

作　　者：苏莉军 闫珺[1] 周丽萍[1] 陈睿[1] SU Lijun;YAN Jun;ZHOU Liping;CHEN Rui(Department of Obstetrics,Henan Provincial People′s Hospital,Zhengzhou 450000,Henan Province,China)

机构地区：[1]河南省人民医院产科,河南郑州450000

出　　处：《新乡医学院学报》2025年第5期379-383,共5页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关项目(编号:LHGJ20200050)。

摘　　要：目的探讨子宫糖基化蛋白(glycodelin-A)、可溶性酪氨酸激酶受体1(sFlt1)水平与子痫前期严重程度及妊娠结局的相关性。方法选择2021年1月至2023年8月在河南省人民医院就诊的子痫前期患者80例为观察组,另选择在本院孕检正常的40例孕妇为对照组。子痫前期患者治疗前、对照组受试者产前抽取清晨空腹静脉血约5 mL,3000 r·min^(-1)离心10~15 min后吸取上清液备用,采用酶联免疫吸附法测定血清glycodelin-A及sFlt1水平。根据发病时间将子痫前期患者分为早发组(n=43)和晚发组(n=37);根据疾病严重程度将子痫前期患者分为轻度组(n=52)和重度组(n=28)。比较各组血清glycodelin-A、sFlt1水平,记录观察组患者不良妊娠结局,采用受试者操作特征(ROC)曲线分析血清glycodelin-A、sFlt1水平对子痫前期患者不良妊娠结局的预测效能。结果观察组患者的血清glycodelin-A水平显著低于对照组,sFlt1水平显著高于对照组(P<0.05)。晚发组和早发组患者的血清glycodelin-A水平显著低于对照组,sFlt1水平显著高于对照组(P<0.05);早发组患者的血清glycodelin-A水平显著低于晚发组,sFlt1水平显著高于晚发组(P<0.05)。轻度组和重度组患者的血清glycodelin-A水平显著低于对照组,sFlt1水平显著高于对照组(P<0.05);重度组患者的血清glycodelin-A水平显著低于轻度组,sFlt1水平显著高于轻度组(P<0.05)。轻度组和重度组患者的不良妊娠结局总发率显著高于对照组,重度组患者不良妊娠结局总发生率显著高于轻度组(P<0.05)。ROC曲线分析结果显示,glycodelin-A预测子痫前期患者不良妊娠结局的曲线下面积(AUC)为0.847[95%置信区间(CI):0.775~0.919],敏感度和特异度分别为78.6%和52.5%;sFlt1预测子痫前期患者不良妊娠结局的AUC为0.823(95%CI:0.743~0.904),敏感度和特异度分别为83.9%和80.0%,二者联合预测子痫前期患者不良妊娠结局的AUC为0.830,敏感度、特异度分别为87.Objective To explore the correlation of glycodelin-A and serum soluble tyrosine kinase receptor 1(sFlt1)levels with the severity of preeclampsia and pregnancy outcomes.Methods A total of 80 preeclampsia patients treated at Henan Provincial People′s Hospital from January 2021 to August 2023 were selected as the observation group,and another 40 women who had normal pregnancy tests at the same hospital were selected as the control group.Five mL of fasting venous blood was collected from both preeclampsia patients before treatment and individuals in the control group before delivery.The blood was centrifuged at 3000 r·min^(-1)for 10 to 15 minutes and the supernatant was collected for further use.Enzyme-linked immunosorbent assay was used to measure the levels of serum glycodelin-A and sFlt1.The preeclampsia patients were divided into an early-onset group(n=43)and a late-onset group(n=37)according to the onset time.The preeclampsia patients were divided into a mild group(n=52)and a severe group(n=28)according to the severity of the disease.The levels of serum glycodelin-A and sFlt1 were compared among each groups.The adverse pregnancy outcomes of patients in the observation group were recorded,and the effect of serum glycodelin-A and sFlt1 levels for predicting adverse pregnancy outcomes in preeclampsia patients was analyzed using receiver operating characteristic(ROC)curves.Results The observation group had significantly lower serum glycodelin-A levels and significantly higher serum sFlt1 levels than those in the control group(P<0.05).The late-onset and early-onset groups had significantly lower serum glycodelin-A levels and significantly higher serum sFlt1 levels than those in the control group(P<0.05).The early-onset group had significantly lower serum glycodelin-A levels and significantly higher serum sFlt1 levels than those in the late-onset group(P<0.05).The mild and severe groups had significantly lower serum glycodelin-A levels and significantly higher serum sFlt1 levels than those in the control group(P<0.

关 键 词：子宫糖基化蛋白 可溶性酪氨酸激酶受体1 子痫前期 严重程度 妊娠结局 相关性 

分 类 号：R714.244[医药卫生—妇产科学]