探讨苁蓉舒痉颗粒对帕金森病小鼠的影响  

Effect of Congrong Shujing Granules on Parkinson′s Disease Mice

作　　者：王志汕 钟佳男 陈凯丽 李茜羽 梁永辉 陈淑娇 陈则钢 陈晓雯 蔡晶 肖绍坚 WANG Zhishan;ZHONG Jianan;CHEN Kaili;LI Xiyu;LIANG Yonghui;CHEN Shujiao;CHEN Zegang;CHEN Xiaowen;CAI Jing;XIAO Shaojian(The Third Affiliated People′s Hospital of Fujian University of Traditional Chinese Medicine,Fujian 350122,China;Beijing University of Chinese Medicine Shenzhen Hospital,Shenzhen 518000,China)

机构地区：[1]福建中医药大学附属第三人民医院,福州350122 [2]北京中医药大学深圳医院,深圳518000

出　　处：《世界中医药》2025年第4期559-567,共9页World Chinese Medicine

基　　金：国家自然科学基金面上项目(82374528);国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函[2023]85号);福建省卫健委科技计划项目(2021zylc33)。

摘　　要：目的:研究苁蓉舒痉颗粒对帕金森病(PD)模型小鼠海马DJ-1蛋白表达、磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/AKT)信号通路的影响,探讨苁蓉舒痉颗粒治疗PD的可能作用机制。方法:将58只C57BL/6J小鼠按照随机数字表法分为空白对照组10只和造模组48只,造模组小鼠经腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)制备PD模型,将造模成功小鼠分为模型组、苁蓉舒痉颗粒低、中、高剂量组,每组11只。造模成功后,苁蓉舒痉颗粒低剂量组、中剂量组、高剂量组分别给予186、371、742 mg/mL苁蓉舒痉颗粒溶液,以0.01 mL/g灌胃,空白对照组、模型组以等量生理盐水灌胃,均1次/d,连续14 d。给药第14 d后开始取材。在给药前和给药第7、14天进行悬挂实验、爬杆实验观察小鼠行为学变化,免疫组织化学法检测小鼠黑质TH阳性细胞和海马DJ-1阳性细胞表达,蛋白质免疫印迹法检测小鼠海马DJ-1、AKT、PI3K、P-AKT、P-PI3K蛋白表达,qPCR法检测小鼠海马DJ-1、PI3K、AKT mRNA表达。结果:造模后及给药第7、14天,与空白对照组比较,模型组悬挂实验评分低(P<0.05);给药第14天,与模型组比较,苁蓉舒痉颗粒组悬挂实验评分高(P<0.05)。给药前及给药第7天各造模组悬挂实验评分皆低于空白对照组(P<0.05),各造模组组间差异无统计学意义;给药第14天,中剂量组、高剂量组与正常组、低剂量组比较悬挂实验评分差异无统计学意义(P>0.05),与模型组比较悬挂实验评分更高(P<0.05),而低剂量组与空白对照组比较悬挂实验评分更低(P<0.05),与模型组比较差异无统计学意义(P>0.05)。与空白对照组比较,模型组爬杆实验评分高(P<0.05);给药第14天,与模型组比较,苁蓉舒痉颗粒高剂量组爬杆实验评分低(P<0.05)。给药前各造模组爬杆实验评分皆高于空白对照组(P<0.05),各造模组组间差异无统计学意义;给药第7天,高剂量组与正常组、低剂量组、中剂量组比Objective:To investigate the effects of Congrong Shujing Granules on the expression of DJ-1 protein and the phosphoinositide 3-kinase/protein kinase B(PI3K/AKT)signaling pathway in the hippocampus of Parkinson′s disease(PD)model mice,and to explore the potential mechanisms underlying its therapeutic effects on PD.Methods:A total of 58 C57BL/6J mice were randomly divided into the blank control group(n=10)and the modeling group(n=48)according to a random number table.The PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP),and the successful model mice were further divided into the model group,low,medium,and high-dose Congrong Shujing Granules groups(n=11 in each group).After modeling,mice in the low,medium,and high-dose Congrong Shujing Granules groups received granule solutions at respective doses(186,371,and 742 mg/mL)by gavage at 0.01 mL/g for 14 days.The blank control group and model group received the same volume of saline,once daily for 14 days.Samples were collected 14 days after drug administration.Behavioral changes were observed through suspension and climbing tests before drug treatment and on day 7 and day 14 after drug treatment.Immunohistochemical analysis was used to detect TH-positive cells in the substantia nigra and DJ-1-positive cells in the hippocampus.Western blot was performed to assess the protein expression of DJ-1,AKT,PI3K,P-AKT,and P-PI3K in the hippocampus,and qPCR was employed to detected the mRNA levels of DJ-1,PI3K,and AKT in the hippocampus.Results:After modeling and on days 7 and 14 after treatment,the suspension test scores in the model group were significantly lower than those in the blank control group(P<0.05).On day 14,the suspension test scores of the Congrong Shujing Granules groups were higher than those in the model group(P<0.05).Before drug treatment and on the 7th day after drug treatment,the suspension test scores of all modeling groups were lower than those of the blank control group(P<0.05),with no significant dif

关 键 词：帕金森病 苁蓉舒痉颗粒 小鼠 行为学 酪氨酸羟化酶 帕金森氏病蛋白7 磷脂酰肌醇3-激酶/蛋白激酶B信号通路 氧化应激 

分 类 号：R277.7[医药卫生—中医学]