王氏保赤丸对腹泻型肠易激大鼠治疗作用及机制  

Therapeutic Effects and Mechanisms of Wang′s Baochi Pills on IBS-D in Rats

作　　者：樊欣钰 邹道远 张梦秋 李慧[1,2] 黄项鸣 陆敏[2,3] FAN Xinyu;ZOU Daoyuan;ZHANG Mengqiu;LI Hui;HUANG Xiangming;LU Min(Nanjing Integrated Traditional Chinese and Western Medicine Hospital,Nanjing University of Chinese Medicine,Nanjing 210028,China;The Third Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210028,China;Nanjing Lishui District Hospital of Traditional Chinese Medicine,Nanjing 211299,China)

机构地区：[1]南京中医药大学附属中西医结合医院,南京210028 [2]南京中医药大学第三临床医学院,南京210028 [3]南京市溧水区中医院,南京211299

出　　处：《世界中医药》2025年第4期568-573,共6页World Chinese Medicine

基　　金：国家自然科学基金面上项目(82272671);精华制药技术开发项目(NH20201101);江苏省干部保健科研课题(BJ20026);江苏省社会发展面上项目(BE2022817)。

摘　　要：目的:观察王氏保赤丸对腹泻型肠易激综合征(IBS-D)模型大鼠脑组织及肠组织中5-HT的表达情况,以及肠道菌群的影响,阐述其治疗IBS-D的部分可能机制,传承并挖掘经方应用。方法:采用番泻叶灌胃联合束缚刺激法建立IBS-D模型。造模成功后,随机数字法分为模型组、痛泻宁组、王氏保赤丸组,加上空白组共4组,每组10只。王氏保赤丸组、痛泻宁组大鼠分别给予中药混悬液灌胃,空白组、模型组给予等体积蒸馏水灌胃,1次/d,持续14 d。观察各组大鼠IBS-D相关症状改善情况、腹壁撤退反射评分(AWR),用免疫组织化学法检测脑肠组织5-羟色胺(5-HT)表达,高通量测序检测肠道菌群变化。结果:与模型组比较,王氏保赤丸组大鼠一般情况改善,大便Bristol评分及2 h排便粒数均不同程度降低(P<0.01),脑、肠组织中5-HT表达降低(P<0.05),且肠道菌群多样性和丰度都有改善。结论:王氏保赤丸能够改善IBS-D模型大鼠腹泻症状及其肠道高敏感状态,降低大鼠脑肠组织中5-HT表达,维持肠道菌群多样性,其机制可能与“微生物-脑-肠”轴有关。Objective:To observe the effect of Wang′s Baochi Pills on the expression of 5-hydroxytryptamine(5-HT)in the brain and intestinal tissues of rats with diarrhea-predominant irritable bowel syndrome(IBS-D)and its impact on the gut microbiota,explore some of the potential mechanisms of Wang′s Baochi Pills in the treatment of IBS-D,and inherit and explore the application of classical formulas.Methods:The IBS-D model was established using a combination of senna leaf gavage and restraint stress.After successful modeling,the rats were randomly divided into the model group,Tongxiening group,Wang′s Baochi Pills group,and a blank control group according to a random number table,with 10 rats in each group.The Wang′s Baochi Pills and Tongxiening groups were administered the respective herbal suspensions,while the blank and model groups received an equal volume of distilled water by gavage,once daily for 14 days.The improvement of IBS-D-related symptoms and abdominal withdrawal reflex(AWR)scores in each group were assessed.The 5-HT expression in brain and intestinal tissues and changes in the gut microbiota were detected using immunohistochemistry and high-throughput sequencing,respectively.Results:Compared with the model group,the Wang′s Baochi Pills group showed significant improvements in general condition,a decrease in Bristol stool scores,and a reduction in the number of stools after 2 hours(P<0.01).The expression of 5-HT in brain and intestinal tissues was significantly reduced(P<0.05),and the diversity and abundance of the gut microbiota improved.Conclusion:Wang′s Baochi Pills can alleviate the diarrhea symptoms and intestinal hypersensitivity in IBS-D model rats,reduce the expression of 5-HT in brain and intestinal tissues,and maintain the diversity of the gut microbiota.The mechanism may be related to the“microbiota-brain-gut”axis.

关 键 词：腹泻型肠易激综合征 王氏保赤丸 腹泻 肠道高敏感 5-羟色胺 肠道菌群 脑-肠轴 微生物-脑-肠轴 

分 类 号：R256.3[医药卫生—中医内科学]