630 nm发光二极管照射脂多糖刺激的THP-1来源巨噬细胞的多组学分析  

The Mult-Omics Analysis of THP-1-Derived Macrophages Stimulated by 630 nm Light Emitting Diodes and Lipopolysaccharide

作　　者：任丹丹 毕婉莹 谢文婷 吴佳欣 宋武琦 张凤民 刘海亮 REN Dandan;BI Wanying;XIE Wenting;WU Jiaxin;SONG Wuqi;ZHANG Fengmin;LIU Hailiang(Wu Lien-Teh Institute,Harbin Medical University,Harbin 150081,China;Department of Microbiology,Harbin Medical University,Harbin 150081,China;Heilongjiang Provincial Key Laboratory of Infection and Immunity,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学伍连德研究所,黑龙江哈尔滨150081 [2]哈尔滨医学大学微生物教研室,黑龙江哈尔滨150081 [3]黑龙江省感染与免疫重点实验室,黑龙江哈尔滨150081

出　　处：《照明工程学报》2025年第2期39-48,共10页China Illuminating Engineering Journal

基　　金：国家重点研发计划资助项目(2022YFB3604705,2017YFB0403805);国家自然科学基金项目(81701573);黑龙江省自然科学基金优秀青年项目(YQ2020H004)。

摘　　要：发光二极管(Light Emitting Diode,LED)作为一种低能量光疗(Low-level Light Therapy,LLLT),已被证实可以治疗肺损伤、肌腱病、神经损伤、类风湿性关节炎等疾病。本研究采用630 nm LED红光作为光源,分析630 nm LED红光照射脂多糖(Lipopolysaccharides,LPS)刺激的人单核细胞白血病细胞(THP-1)来源巨噬细胞的作用机制。用LPS处理THP-1细胞,并使用强度为28.8 J/cm^(2)的630 nm LED红光照射,随后检测促炎因子CXCL9、CXCL10、CXCL11的mRNA表达水平及采用非标定量蛋白质组学及磷酸化蛋白质组学分析方法筛选差异表达蛋白并进行基因本体论(Gene Ontology,GO)和KEGG富集;通过STRING在线网站(https://cn.string-db.org/)和Cytoscape软件分析蛋白质互作情况。结果显示,经LED照射的THP-1细胞CXCL9、CXCL10、CXCL11的mRNA表达水平较对照组显著降低。蛋白质组学结果显示,630 nm LED红光照射巨噬细胞后,23个差异表达蛋白能够显著富集在转录调节复合体、病毒防御反应的调控等通路。磷酸化蛋白质组学结果显示,有178个位点的修饰水平能够显著富集在核斑点、钙粘蛋白结合、蛋白信号转导、蛋白质定位的正向调控、肌动蛋白细胞骨架等通路中。结合多组学分析,我们推测630 nm LED红光可能是通过多种途径来实现抗炎作用的。LED,as a form of Low-Level Light Therapy(LLLT),have been proven effective in treating diseases such as lung injury,tendinopathy,nerve damage,and rheumatoid arthritis.This study uses 630 nm LED red light as the light source to analyze the mechanism of action of 630 nm LED red light on macrophages derived from human monocytic leukemia cells(THP-1)stimulated with lipopolysaccharides(LPS).THP-1 cells were treated with LPS and irradiated with 630 nm LED red light at an intensity of 28.8 J/cm^(2).The mRNA expression levels of pro-inflammatory factors CXCL9,CXCL10 and CXCL11 were then measured.Differentially expressed proteins were screened using non-quantitative proteomics and phosphoproteomics analysis methods.Gene Ontology(GO)and KEGG enrichment analyses were performed.Through STRING online database(https://cn.string-db.org/)and Cytoscape software is used to analyze protein interactions.The results showed that the mRNA expression levels of CXCL9,CXCL10 and CXCL11 in the LED-irridated THP-1 cells were significantly reduced compared to the control group.Proteomic analysis revealed that after 630 nm LED red light irradiation of macrophages,23 differentially expressed proteins were significantly enriched in pathways such as transcriptional regulation complexes and viral defense response regulation.Phosphoproteomics results showed that the modification levels of 178 sites were significantly enriched in pathways such as nuclear spots,cadherin binding,protein signal transduction,positive regulation of protein localization,and actin cytoskeleton.Combined with multi-omics analysis,we speculate that the 630 nm LED red light may exert anti-inflammatory effects through multiple pathways.

关 键 词：发光二极管 THP-1 630 nm红光 蛋白质组学 磷酸化蛋白质组学 光生物调节 

分 类 号：TM923[电气工程—电力电子与电力传动]