短程运输温度对牛卵母细胞体外胚胎生产的影响  

Effect of short-term transportation temperature on in vitro embryo production from bovine oocytes

作　　者：韩修远 张利军 丁红波 姜宏星 王洪亮[1] 张震 陈保君 李伟[1] 韩志强 HAN Xiuyuan;ZHANG Lijun;DING Hongbo;JIANG Hongxing;WANG Hongliang;ZHANG Zhen;CHEN Baojun;LI Wei;HAN Zhiqiang(Institute of Animal Husbandry and Veterinary Medicine,Heilongjiang Academy of Land Reclamation Sciences,Harbin 150038,China;Beian Agricultural Reclamation Changxin Ranch Cooperative,Beian 164000,China;Beidahuang Group Heilongjiang Changshui River Agricultural Co.Ltd.,Beian 164000,China)

机构地区：[1]黑龙江省农垦科学院畜牧兽医研究所,黑龙江哈尔滨150038 [2]北安农垦长鑫牧场专业合作社,黑龙江北安164007 [3]北大荒集团黑龙江长水河农场有限公司,黑龙江北安164007

出　　处：《黑龙江动物繁殖》2025年第2期35-39,共5页Heilongjiang journal of animal reproduction

基　　金：北大荒农垦集团有限公司种业创新发展项目“牛胚胎生物工程技术”(2022ZY0102-02-03);财政部和农业农村部国家现代农业产业技术体系项目(CARS-37)。

摘　　要：少于6 h的短程运输是牛卵巢采集后体外胚胎生产(IVP)的核心环节,温度选择将直接影响卵母细胞发育。试验在4~6 h短程运输条件下,对比15℃与37℃温度条件对卵母细胞成熟及胚胎发育的影响。结果表明:37℃组卵母细胞成熟率与囊胚形成率显著高于15℃组(P<0.05),复温后37℃组HSP70表达量更高,且复温4 h后37℃组卵母细胞的丙二醛(MDA)浓度显著低于15℃组(P<0.05)。说明短程运输中37℃与15℃相比更有利于维持生理代谢与结构完整性,能够显著提升卵母细胞质量,推荐作为标准化操作温度。Short-term transportation(<6 h)of bovine ovaries is a critical step in post-collection in vitro embryo production(IVP),where temperature setting directly influences oocyte developmental competence.This study simulated 4-6 h transportation conditions to compare the effects of 15℃and 37℃on oocyte maturation and embryonic development.Results demonstrated that the significantly higher oocyte maturation rates[(83.2±3.1)%vs.(64.7±4.5)%,P<0.05]and blastocyst formation rates[(42.6±2.8)%vs.(28.3±3.4)%,P<0.05]were observed in the 37℃transportation group,compared to the 15℃group.Post-rewarming analysis revealed elevated HSP70 expression in the 37℃group,along with lower malondialdehyde(MDA)concentrations in oocytes after 4 h of temperature recovery[(1.8±0.3)nmol/mg vs.(3.2±0.4)nmol/mg,P<0.05].These findings indicated that a better physiological metabolism and structural integrity during short-term transportation for collected oocytes could be achieved with the transportation temperature of 37℃,compared to 15℃.Therefore,37℃is recommended as the standardized operational temperature for short-term transportation of oocytes in IVP.

关 键 词：体外胚胎生产 运输 温度 卵巢 卵母细胞成熟率 囊胚 

分 类 号：S823.36[农业科学—畜牧学]