一种低密度猪精液稀释粉使用效果分析  

Efficacy evaluation of a low-density porcine semen diluent

作　　者：刘琳[1] 赵光蒲 潘红双 张雅雯 王尔雅 靳晓静 卞佳怡 李明[1] 赵姝灿 李新建[1] 杨峰[1] LIU Lin;ZHAO Guangpu;PAN Hongshuang;ZHANG Yawen;WANG Erya;JIN Xiaojing;BIAN Jiayi;LI Ming;ZHAO Shucan;LI Xinjian;YANG Feng(College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学动物科技学院,河南郑州450046

出　　处：《黑龙江动物繁殖》2025年第2期44-47,共4页Heilongjiang journal of animal reproduction

基　　金：牧原集团校企合作横向课题"猪精液高效保存技术研发与高繁机制解析"(30501638)。

摘　　要：为了解决猪精液高倍稀释后难以保存的问题,试验探索了在低密度猪精液稀释粉(HNAU)中添加牛血清白蛋白(BSA)对猪精液低密度(1亿/60 mL、3亿/60 mL、4亿/60 mL、5亿/60 mL)保存效果的影响。HNAU不添加BSA为试验1组,HNAU添加BSA为试验2组,商业稀释粉V为对照组。结果表明:与对照组和试验1组相比,试验2组在精子密度为1亿/60 mL时精子保存时间显著提升(P<0.05);在精子密度为3亿/60 mL、4亿/60 mL时保存72 h后,试验2组精子活力显著高于对照组(P<0.05),且96 h后仍维持>70%的活力;对照组和试验2组猪精液稀释至3亿/60 mL配种后配妊率均大于95%,两组间无显著差异(P>0.05)。说明HNAU添加BSA可显著提高低密度猪精液保存效果。To address the challenges in preserving highly diluted porcine semen,this study investigated the effects of supplementing bovine serum albumin(BSA)in a low-density semen extender(HNAU)on preservation efficacy at diluted sperm concentrations of 100 million(1×10^(8)),300 million(3×10^(8)),400 million(4×10^(8)),and 500 million(5×10^(8))spermatozoa per 60 mL.Experimental groups included HNAU without BSA(group 1),HNAU with BSA(group 2),and a commercial extender V as the control.Results demonstrated:At 1×10^(8)/60 mL,group 2 exhibited significantly prolonged sperm preservation duration compared to both the control and group 1(P<0.05).For 3×10^(8)and 4×10^(8)/60 mL concentrations,group 2 maintained superior motility(>70%)after 96 h preservation,with significantly higher viability than the control at 72 h(P<0.05).No significant difference(P>0.05)in conception rates(>95%)was observed between the control and group 2 when inseminated with 3×10^(8)/60 mL diluted semen.These results indicate that BSA supplementation in HNAU significantly enhances low-density porcine semen preservation performance,providing a viable strategy for improving sperm survival in diluted artificial insemination protocols.

关 键 词：猪精液 高倍稀释 低密度精液 精液保存 低剂量输精 

分 类 号：S826.34[农业科学—畜牧学]