Box-Behnken法优化蝇蛹提取蛋白质工艺和制备甲壳素  

Optimization of protein extracted from fly pupae using Box-Behnken method and preparation of chitin

作　　者：圣志存 冯欣 王婧[1] SHENG Zhicun;FENG Xin;WANG Jing(Jiangsu Agri-animal Husbandry and Vocational College,Taizhou,Jiangsu Province 225300,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《中国饲料》2025年第9期51-57,共7页China Feed

基　　金：国家自然科学基金青年基金(31902317);江苏省青蓝工程项目(苏教师函2022(29)号);江苏农牧科技职业学院科研项目(NSF2023ZR16)。

摘　　要：为探索昆虫资源高值化利用,本试验以蝇蛹为原料提取蛋白质和制备甲壳素。采用碱提法、Box-Behnken设计、响应面优化脱蛋白工艺酸沉提取蛋白质,酸浸法脱盐和氧化法脱色制备甲壳素,同时红外光谱分析进行表征。结果表明:碱提法提取蝇蛹蛋白质最佳条件为NaOH浓度10.5%,反应温度100℃,反应时间3.2 h,料液比1:20,蛋白质提取率为(50.26±0.20)%。酸浸法脱盐浓度为2 mol/L HCl;氧化法脱色条件为1%NaClO。红外光谱分析发现,制备甲壳素显示典型特征吸收峰,甲壳素提取率为18.89%。本研究可为蝇蛹或其他昆虫资源提取蛋白质和甲壳素提供参考。In order to explore the high-value utilization of insects,fly pupae was used as raw material for extracting protein and preparing chitin in this experiment.The deproteinization process was optimized using Box-Behnken design of response surface method for extracting protein by alkaline extraction and acid precipitation,while the desalination and decolorization process was performed by acid leaching and oxidation method for preparing chitin,respectively,and the characterization was also conducted by infrared spectrum analysis.The results showed that the optimal conditions by alkaline extraction for extracting protein were as follows:NaOH concentration of 10.5%,reaction temperature of 100℃,reaction time of 3.2 h,solid-liquid ratio of 1:20,and the extracted yield of protein was(50.26±0.20)%.The optimal desalination concentration by acid leaching method was 2 mol/L HCl.The decolorization condition by oxidation method was 1%NaClO.Infrared spectrum analysis of prepared chitin showed a typical characteristic absorption,and the final yield of chitin was 18.89%.This study could provide a reference for extracting protein and chitin from fly pupae or other insects resource.

关 键 词：蝇蛹 蛋白质 响应面法 脱盐 脱色 甲壳素 

分 类 号：S816.7[农业科学—饲料科学]