响应面法优化提取黔产博落回生物碱及其分离纯化研究  

Optimization of extraction and separation purification of bioalkaloids from Guizhou Macleaya cordata(Willd.)R.Br.by response surface methodology

作　　者：戴天伦 代应会 陈洪声 张林 丰秋兰 DAI Tianlun;DAI Yinghui;CHEN Hongsheng;ZHANG Lin;FENG Qiulan(Institute of Natural Resources,Guizhou Institute of Chemical Technology,Guiyang,Guizhou Province 550025,China)

机构地区：[1]贵州省化工研究院自然资源所,贵州贵阳550025

出　　处：《中国饲料》2025年第9期64-71,共8页China Feed

基　　金：贵州科学院青年基金项目(黔科院J字[2023]5号);贵州省科技计划项目(黔科合服企[2022]002);贵州省科技计划项目(黔科合成果[2022]重点004)。

摘　　要：为开发新型生物碱添加剂,本试验以博落回果荚为原料,通过响应面法优化乙醇提取博落回总生物碱的工艺条件,并结合大孔树脂法进行分离纯化。结果表明:最佳提取工艺为:乙醇浓度70%,料液比1:16(g/mL),提取时间2 h,提取温度70℃。经3次平行试验得到黔产博落回生物碱平均提取得率为11.98%,其理论预测值为12.02%,相对误差0.33%,表明该模型可靠。优选出HPD100为洗脱树脂,并采用梯度洗脱得到四种纯化的成分,通过HPLC对纯化后的生物碱纯度进行鉴定,结果显示纯化后的总生物碱纯度超过70%,其中盐酸血根碱为68.2%,盐酸白屈菜红碱为8.5%。研究表明,乙醇法能有效提取博落回中的生物碱,大孔树脂分离使得生物碱纯度提高,丰富了博落回生物碱利用的途径。The experiment aimed to develop new types of alkaloid additives.Macleaya cordata(Willd.)R.Br.fruit pods were used as raw materials,the process conditions for ethanol extraction of total alkaloids from Boluohui were optimized by response surface methodology,and combined with macroporous resin method for separation and purification.The results showed that the optimal extraction process was as follows:ethanol concentration of 70%,solid-liquid ratio of 1:16(g/mL),extraction time of 2 hours,and extraction temperature of 70℃.After three parallel experiments,it was found that the average extraction rate of alkaloids from Macleaya cordata(Willd.)R.Br.in Guizhou was 11.98%,with a theoretical prediction value of 12.02%and a relative error of 0.33%,indicating the reliability of the model.HPD100 was selected as the elution resin,and four purified components were obtained by gradient elution.The purity of the purified alkaloids was identified by HPLC,and the results showed that the total purity of the purified alkaloids exceeded 70%,including 68.2%of sanguine hydrochloride and 8.5%of berberine hydrochloride.Research had shown that the ethanol method could effectively extract alkaloids from Macleaya cordata,and macroporous resin separation improves the purity of alkaloids,enriching the pathways for the utilization of Macleaya cordata(Willd.)R.Br.alkaloids.

关 键 词：响应面法 博落回 生物碱 大孔树脂 分离纯化 

分 类 号：S816.7[农业科学—饲料科学]