Tespa1在慢性鼻窦炎伴鼻息肉中的表达及作用研究  

The expression and function of Tespa1 in chronic rhinosinusitis with nasal polyps

作　　者：张广玲 陈兴雪 武天义 孙占伟 王卫卫 李世超 王广科 ZHANG Guangling;CHEN Xingxue;WU Tianyi;SUN Zhanwei;WANG Weiwei;LI Shichao;WANG Guangke(Department of Otorhinolaryngology&Head and Neck Surgery,Zhengzhou University People's Hospital/Henan Province People's Hospital,Zhengzhou 450003,Henan,China)

机构地区：[1]郑州大学人民医院/河南省人民医院耳鼻咽喉头颈外科,河南郑州450003

出　　处：《山东大学耳鼻喉眼学报》2025年第2期35-42,50,共9页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：河南省2022科技发展计划(222102310203)。

摘　　要：目的 检测胸腺细胞表达的阳性选择相关蛋白1(thymocyte expressed, positive selection associated 1, Tespa1)在鼻息肉及鼻腔黏膜中的表达及表达差异,并探讨Tespa1在CRSwNP中的作用及其与肥大细胞的关系。方法 研究纳入慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with Nasal Polyps, CRSwNP)患者29例、慢性鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps, CRSsNP)患者20例、对照组患者18例。应用实时定量聚合酶链反应(quantitative real-time PCR, qPCR)、免疫组织化学法(immunohistochemistry, IHC)、酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)、和苏木精-伊红(hematoxylin-eosin staining, H&E)染色,检测鼻腔黏膜及鼻息肉中Tespa1表达及分布,并分析了其与肥大细胞、IgE、IL-4及IL-13之间的相关性。结果 qPCR结果显示,CRSwNP中Tespa1 mRNA表达水平较对照组及CRSsNP组均显著降低(Z=2.42,P=0.046;Z=-3.38,P=0.002)。IHC结果显示,与对照组相比,Tespa1在鼻息肉的上皮和间质低表达,胰蛋白酶阳性的肥大细胞在鼻息肉中表达加(t=10.14,P<0.001)。鼻息肉中Tespa1阳性细胞数与胰蛋白酶阳性肥大细胞数呈负相关(r=-0.710,P=0.006)。ELISA结果显示,组织中Tespa1水平远高于其在血清中的水平,组织Tespa1水平与IgE、IL-4、IL-13水平呈负相关(r=-0.682,P=0.002;r=-0.691,P=0.002;r=-0.539,P=0.021)。结论 Tespa1在CRSwNP患者鼻息肉组织中低表达,与IgE、IL-4及IL-13表达水平负相关,并可能通过肥大细胞发挥作用。本研究为理解CRSwNP的病理机制提供了新的视角,并可能为未来的诊断和治疗提供新的分子靶标。Objective To determine the expression and expression differences of thymocyte-expressed,positive selection associated 1(Tespa1)in nasal polyps and nasal mucosa and to investigate the role of Tespa1 in CRSwNP and its relationship with mast cells.Methods Twenty-nine patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and 20 patients with chronic rhinosinusitis without nasal polyps(CRSsNP)were included in the study,18 cases of controls.Quantitative real-time PCR(qPCR),immunohistochemistry(IHC),enzyme-linked immunosorbent assay(ELISA),and hematoxylin-eosin(H&E)staining were used to detect the expression and distribution of Tespa1 in nasal mucosa and nasal polyps,and its correlation with mast cells,IgE,IL-4,and IL-13 was analyzed.Results The qPCR results showed that the expression level of Tespa1 mRNA in CRSwNP was significantly lower than that in both control and CRSsNP groups(Z=2.42,P=0.046;Z=-3.38,P=0.002).The IHC results showed that Tespa1 was lowly expressed in the epithelium and mesenchyme of nasal polyps compared with the control group,and the expression of tryptase-positive mast cells was upregulated in nasal polyps(t=10.14,P<0.001).The number of Tespa1-positive cells in nasal polyps was negatively correlated with the number of trypsin-positive mast cells(r=-0.710,P=0.006).The ELISA results showed that the tissue Tespa1 level was much higher than its level in serum,and the tissue Tespa1 level was negatively correlated with the levels of IgE,IL-4,and IL-13(r=-0.682,P=0.002;r=-0.682,P=0.002;r=-0.691,P=0.002;r=-0.539,P=0.021).Conclusions Tespa1 is low expressed in nasal polyp tissues of CRSwNP patients,negatively correlated with the expression levels of IgE,IL-4 and IL-13,and may act through mast cells.This study provides a new perspective for understanding the pathomechanism of CRSwNP and may provide new molecular targets for future diagnosis and treatment.

关 键 词：慢性鼻窦炎伴鼻息肉 胸腺细胞表达的阳性选择相关蛋白1 肥大细胞 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]