桉树WOX转录因子家族全基因组鉴定及比较分析  

Genome-wide Identification and Analysis of WOX Transcription Factor Family in Eucalyptus

作　　者：刘家璇 黄楚涵 欧阳乐军 王超洪 柯梓婷 沈超 LIU Jiaxuan;HUANG Chuhan;OUYANG Lejun;WANG Chaohong;KE Ziting;SHEN Chao(College of Life and Geographic Sciences,Kashi University,Kashi Xinjiang 844008,China;Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region,Kashi University,Kashi Xinjiang 844008,China;College of Biology and Food Engineering,Guangdong University of Petrochemical Technology,Maoming Guangdong 525099,China)

机构地区：[1]喀什大学生命与地理科学学院,新疆喀什844008 [2]喀什大学新疆帕米尔高原生物资源与生态重点实验室(喀什),新疆喀什844008 [3]广东石油化工学院生物与食品工程学院,广东茂名525099

出　　处：《长沙大学学报》2025年第2期48-57,共10页Journal of Changsha University

基　　金：国家自然科学基金项目“miR396应答新型细胞分裂素PBU调控尾巨桉体胚高频发生的分子机制研究”(32071780);广东省乡村振兴科技专项“茂名市热带特色作物种质资源挖掘与生物育种技术创新中心”(2021S0074);广东省大学生省级创新项目“TCPS应答PBU调控尾巨桉不定芽高频发生的机理研究”(24B113)。

摘　　要：WUSCHEL相关同源盒(WUSCHEL-related homeobox,简称WOX)基因家族是植物特有的转录因子家族,在植物的生长发育及器官形成与分化过程中发挥着重要作用。为了比较尾巨桉与巨桉WOX基因的差异,研究采用生物信息学方法分别在尾巨桉和巨桉基因组中鉴定出8个WOX基因家族成员。分析显示,这些WOX成员的氨基酸序列长度在111~377个氨基酸残基之间,其二级结构主要由α螺旋和无规则卷曲组成,均表现为亲水性蛋白。亚细胞定位预测结果表明,WOX基因均定位于细胞核内。系统进化分析显示,WOX基因家族可分为远古支、中间支和现代支三个进化分支。保守基序分析发现,除尾巨桉的Eug03G0133100外,其余尾巨桉和巨桉WOX蛋白均含有Motif1,表明该基序在进化过程中高度保守。染色体定位分析表明,尾巨桉的WOX基因分布于6条染色体上,而巨桉WOX基因分布于7条染色体上,并且二者共有7对同源基因。基于尾巨桉愈伤组织不同发育时期的转录组数据分析发现,在愈伤组织形成的四个阶段,有6个WOX基因具有表达活性。其中,Eug01G0190000在白色愈伤和红色愈伤阶段表现出显著的差异表达。qRT-PCR分析结果显示,在1 mg/L PBU处理下,WOX基因的表达显著上调,并有效促进愈伤组织的分化,尤以Eug02G0065500的表达量最高。综合分析表明,这两个基因可能作为候选基因,参与尾巨桉愈伤组织的形成和分化。The WUSCHEL-related homeobox(WOX)gene family is a plant-specific transcription factor family that plays a crucial role in plant growth,development,and organ formation and differentiation.To compare the differences in the WOX genes between E.urophylla×E.grandis(hybrid Eucalyptus)and E.grandis,bioinformatics methods were used to identify 8 WOX gene family members in the genomes of both species.The analysis reveals that the amino acid sequences of these WOX members ranged from 111 to 377 residues,with secondary structures primarily consisting ofα-helices and random coils,and all of them were hydrophilic proteins.Subcellular localization predictions indicated that the WOX proteins were located in the nucleus.Phylogenetic analysis showed that the WOX gene family could be divided into three evolutionary branches:ancient,intermediate,and modern.Conserved motif analysis reveals that,except for Eug03G0133100 in E.urophylla×E.grandis,all other WOX proteins in both E.urophylla×E.grandis and E.grandis contained Motif1,suggesting that this motif is highly conserved during evolution.Chromosomal localization analysis shows that the WOX genes of E.urophylla×E.grandis were distributed across 6 chromosomes,while the WOX genes of E.grandis were distributed across 7 chromosomes,with 7 pairs of homologous genes shared between the two species.Based on transcriptomic data from different developmental stages of callus tissue in E.urophylla×E.grandis,six WOX genes were found to be actively expressed during the formation of callus tissue in the four stages.Among them,Eug01G0190000 exhibited significant differential expression between white and red callus stages.qRT-PCR analysis revealed that treatment with 1 mg/L PBU significantly upregulated the expression of WOX genes and effectively promoted callus differentiation,with Eug02G0065500 showing the highest expression levels.Comprehensive analysis suggests that these two genes could serve as candidate genes involved in the formation and differentiation of callus tissue in E.urophylla�

关 键 词：尾巨桉 巨桉 WOX转录因子家族 愈伤组织 

分 类 号：S792.39[农业科学—林木遗传育种]