复方黄芪颗粒对小鼠抗疲劳的作用及其机制研究  

Study on the anti-fatigue effect of compound Huangqi granules on mice and its mechanism

作　　者：张志强 张鹏 王松萍 刘江[1] 王晓聪 ZHANG Zhiqiang;ZHANG Peng;WANG Songping;LIU Jiang;WANG Xiaocong(Department of Pharmacy,Shenzhen Luohu People’s Hospital,Shenzhen 518001,China;Department of Pharmacy,Shenzhen Luohu District Chinese Medicine Hospital,Shenzhen 518009,China)

机构地区：[1]深圳市罗湖区人民医院药学部,广东深圳518001 [2]深圳市罗湖区中医院药学部,广东深圳518009

出　　处：《广州医药》2025年第4期509-515,共7页Guangzhou Medical Journal

基　　金：广东省医院协会医院药学科研专项基金项目(YXKY202215);广东省医院药学研究基金项目(2021A16);深圳市医疗卫生三名工程项目(NO:SZSM202301035)。

摘　　要：目的探讨复方黄芪颗粒(CHG)的抗疲劳作用及其机制。方法48只雄性BALB/C小鼠随机分为空白对照组、低剂量(9.1 g/kg)、中剂量(18.2 g/kg)、高剂量(27.3 g/kg)CHG 3个试验组,每组12只。试验组给予不同剂量的复方黄芪颗粒溶液灌胃,空白对照组小鼠给予等体积生理盐水。给药30 d后,检测小鼠体内相关指标变化,观察其抗疲劳作用并分析相关机制。结果与空白对照组相比,试验组小鼠体质量差异无统计学意义(P>0.05),小鼠力竭游泳时间及转棒耐力时间均明显延长(P<0.01),血尿素氮(BUN)、乳酸脱氢酶(LDH)、丙二醛(MDA)水平明显降低(P<0.01),肝糖原和肌糖原水平升高(P<0.05),超氧化物歧化酶(SOD)活性升高(P<0.01)。体外抗氧化试验表明CHG以剂量依赖性方式清除2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基。当CHG质量浓度为100.0000 mg/mL时,CHG对DPPH自由基清除能力可达85.0303%。当CHG质量浓度为25.0000 mg/mL时,CHG对ABTS自由基清除能力可达96.3572%。结论CHG具有抗疲劳的作用,其作用机制可能与抗氧化作用相关。Objective To investigate the anti-fatigue effects of compound Huangqi granules(CHG)and its mechanism.Methods Forty-eight male BALB/C mice were randomly divided into blank control group,9.1,18.2,27.3 g/kg CHG group(test groups).The test groups received different concentrations of CHG solution by gavage,and the blank control group mice were given equal volume saline.After 30 days of administration,the mice were tested,meanwhile the anti-fatigue effect and mechanism were investigated.Results Compared with blank control group,there was no significant difference in body weight(P>0.05).The exhaustive swimming time and rod turning endurance time of mice were significantly prolonged(P<0.01).The serum levels of blood urea nitrogen,lactate dehydrogenase and malondialdehyde were significantly decreased(P<0.01),while the liver and muscle glycogen levels(P<0.05)and superoxide dismutase activity were increased(P<0.01).In vitro antioxidant tests showed that CHG can remove(1,1-Diphenyl-2-picrylhydrazyl,ABTS)and(2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid,DPPH)free radicals in a dose-dependent manner.When the CHG concentration is 100 mg/mL,the DPPH free radical scavenging ability of CHG can reach 85.0303%.When the CHG concentration was 25 mg/mL,the scavenging ability of CHG to ABTS free radicals reached 96.3572%.Conclusions CHG has anti-fatigue effect,and its mechanism may be related to anti-oxidation effect.

关 键 词：复方黄芪颗粒 抗疲劳 抗氧化 机制 

分 类 号：R285.5[医药卫生—中药学]