积雪草提取物体外舒缓和修复作用研究  

Study on the soothing and repairing effects of Centella asiatica(L.)Urban extract in vitro

作　　者：黄晓燕[1] 胡敬[1] 耿长安[1] 张雪梅[1] 李天泽 陈纪军[1] HUANG Xiaoyan;HU Jing;GENG Changan;ZHANG Xuemei;LI Tianze;CHEN Jijun(State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201,China)

机构地区：[1]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650201

出　　处：《中国化妆品科学》2025年第1期31-37,共7页

摘　　要：以研究积雪草提取物对皮肤的舒缓和修复作用为目的,通过体外清除2,2-二苯基-1-苦酰基(DPPH)、对H2O2损伤的人皮肤成纤维细胞(HSF)和人永生化表皮细胞(HaCaT)的保护作用和促HaCaT细胞分泌Ⅰ型胶原蛋白(ColⅠ)和透明质酸(HA)活性,评价其体外活性。结果表明,积雪草提取物在200.0μg/mL浓度时,对两个皮肤细胞安全无毒性,对DPPH自由基清除率为21.8%;25.0μg/mL浓度时,对H2O2损伤的HSF和HaCaT细胞修复率分别为34.9%和23.5%,对ColⅠ分泌的促进率为29.5%;对HA分泌促进率为20.0%。表明积雪草提取物兼具代表性成分(积雪草酸、羟基积雪草酸、积雪草苷、羟基积雪草苷)活性,在体外能有效抗氧化、促进细胞分泌细胞外基质,具有效舒缓和修复皮肤功效。Objective:To investigate the soothing and repairing the skin effects of Centella asiatica(L.)Urban extract in vitro.The extract was evaluated by its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl(DPPH)radicals,protect human skin fibroblasts(HSF)and human keratinocyte cell(HaCaT)from H2O2-induced damage,and stimulate the secretion of collagen type I(ColⅠ)and hyaluronic acid(HA)by HaCaT cells.Results:The Centella asiatica extract was safe to both HSF and HaCaT at the concentration of 200.0μg/mL.At the concentration of 25.0μg/mL,it had a DPPH radical scavenging rate of 21.8%,the repair rates for H2O2-damaged HSF and HaCaT cells were 34.9%and 23.5%,and it also promoted the secretion of type I collagen by 29.5%and hyaluronic acid by 20.0%.Conclusion:Centella asiatica extract,with the activity of its representative components(asiatic acid,madecassic acid,asiaticoside,and madecassoside),has been shown to effectively resist oxidation,promote cells to secrete extracellular matrix,and provide soothing and skin-repairing benefits in vitro.

关 键 词：积雪草 舒缓 修复 皮肤成纤维细胞(HSF) 人永生化表皮细胞(HaCaT) 

分 类 号：TQ630[化学工程—精细化工]